On a cold night: transcriptomics of grapevine flower unveils signal transduction and impacted metabolism - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2019

On a cold night: transcriptomics of grapevine flower unveils signal transduction and impacted metabolism

Abstract

Low temperature is a critical environmental factor limiting plant productivity, especially in northern vineyards. To clarify the impact of this stress on grapevine flower, we used the Vitis array based on Roche-NimbleGen technology to investigate the gene expression of flowers submitted to a cold night. Our objectives were to identify modifications in the transcript levels after stress and during recovery. Consequently, our results confirmed some mechanisms known in grapes or other plants in response to cold stress, notably, (1) the pivotal role of calcium/calmodulin-mediated signaling; (2) the over-expression of sugar transporters and some genes involved in plant defense (especially in carbon metabolism), and (3) the down-regulation of genes encoding galactinol synthase (GOLS), pectate lyases, or polygalacturonases. We also identified some mechanisms not yet known to be involved in the response to cold stress, i.e., (1) the up-regulation of genes encoding G-type lectin S-receptor-like serine threonine-protein kinase, pathogen recognition receptor (PRR5), or heat-shock factors among others; (2) the down-regulation of Myeloblastosis (MYB)-related transcription factors and the Constans-like zinc finger family; and (3) the down-regulation of some genes encoding Pathogen-Related (PR)-proteins. Taken together, our results revealed interesting features and potentially valuable traits associated with stress responses in the grapevine flower. From a long-term perspective, our study provides useful starting points for future investigation.
Fichier principal
Vignette du fichier
2019_Sawicki_IJMS_1.pdf (7.09 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02618118 , version 1 (25-05-2020)

Licence

Identifiers

Cite

Mélodie Sawicki, Marine Rondeau, Barbara Courteaux, Fanja Rabenoelina, Gea Guerriero, et al.. On a cold night: transcriptomics of grapevine flower unveils signal transduction and impacted metabolism. International Journal of Molecular Sciences, 2019, 20 (5), pp.1130. ⟨10.3390/ijms20051130⟩. ⟨hal-02618118⟩
61 View
43 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More