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Protective Effect of an Avocado Peel Polyphenolic Extract Rich in Proanthocyanidins on the Alterations of Colonic Homeostasis Induced by a High-Protein Diet

Abstract : Avocado peel, a byproduct from the avocado pulp industry, is a promising source of polyphenolic compounds. We evaluated the effect of a proanthocyanidin-rich avocado peel polyphenol extract (AvPPE) on the composition and metabolic activity of human fecal microbiota cultured for 24 h in a bioreactor in the presence of high protein (HP) amounts and the effect of the resulting culture supernatants (CSs) on HT-29Glc(-/+) and Caco-2 cells. AvPPE decreased the HP-induced production of ammonia, H2S, propionate, and isovalerate and increased that of indole and butyrate. Microbiota composition was marginally affected by HP, whileAvPPE increased the microorganisms/abundance of phylum Actinobacteria, families Coriobacteriaceae and Ruminococcaceae, and genus Faecalibacterium. AvPPE failed to prevent the HP-induced decrease of HT-29Glc(-/+) cell viability and energy efficiency but prevented the HP-induced alterations of barrier function in Caco-2 cells. Additionally, the genotoxic effect of the CSs upon HT-29Glc(-/+) was attenuated by AvPPE. Therefore, AvPPE may be considered as a promising product for improving colonic homeostasis.
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https://hal.inrae.fr/hal-02618579
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Submitted on : Monday, May 25, 2020 - 3:02:41 PM
Last modification on : Friday, June 4, 2021 - 2:40:14 PM

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María José Cires, Paola Navarrete, Edgar Pastene, Catalina Carrasco-Pozo, Rodrigo Valenzuela, et al.. Protective Effect of an Avocado Peel Polyphenolic Extract Rich in Proanthocyanidins on the Alterations of Colonic Homeostasis Induced by a High-Protein Diet. Journal of Agricultural and Food Chemistry, American Chemical Society, 2019, 67 (42), pp.11616-11626. ⟨10.1021/acs.jafc.9b03905⟩. ⟨hal-02618579⟩

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