Skip to Main content Skip to Navigation
Journal articles

Estrogenic Compounds or Adiponectin Inhibit Cyclic AMP Response to Human Luteinizing Hormone in Mouse Leydig Tumor Cells

Abstract : Mouse Leydig Tumor cells (mLTC), transiently expressing cAMP-dependent luciferase, were used to study the influence of sexual steroids and of adiponectin (ADPN) on the cAMP response to luteinizing hormones (LH). While testosterone and progesterone had no significant effect, several molecules with estrogenic activity (17 beta -estradiol, ethynylestradiol, and bisphenol A) provoked a decrease in intracellular cyclic AMP accumulation under 0.7 nM human LH stimulation. Adiponectin exhibited a bimodal dose-effect on LH response: synergistic between 2-125 ng/mL and inhibitory between 0.5-5 mu g/mL. In brief, our data indicate that estrogens and ADPN separately exert rapid (<1 h) inhibitory and/or synergistic effects on cAMP response to LH in mLTC-1 cells. As the inhibitory effect of each estrogenic molecule was observed after only 1-h preincubation, it might be mediated through the G protein-coupled estrogen receptor (GPER) membrane receptor, but this remains to be demonstrated. The synergistic effect with low concentrations of ADPN with human Luteinizing Hormone (hLH) was observed with both fresh and frozen/thawed ADPN. In contrast, the inhibitory effect with high concentrations of ADPN was lost with frozen/thawed ADPN, suggesting deterioration of its polymeric structure.
Document type :
Journal articles
Complete list of metadata

Cited literature [56 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02619081
Contributor : Migration Prodinra <>
Submitted on : Monday, May 25, 2020 - 4:12:12 PM
Last modification on : Monday, April 12, 2021 - 10:10:05 AM

File

2019_Nguyen_Biology_8_2_45_ope...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Thi-Mong-Diep Nguyen, Danièle Klett, Yves Combarnous. Estrogenic Compounds or Adiponectin Inhibit Cyclic AMP Response to Human Luteinizing Hormone in Mouse Leydig Tumor Cells. Biology, MDPI 2019, 8 (2), ⟨10.3390/biology8020045⟩. ⟨hal-02619081⟩

Share

Metrics

Record views

49

Files downloads

101