Choice of protocol for the in vivo bioassay of equine chorionic gonadotropin (eCG/PMSG) in immature female rats - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Theriogenology Year : 2019

Choice of protocol for the in vivo bioassay of equine chorionic gonadotropin (eCG/PMSG) in immature female rats

Abstract

Equine Chorionic Gonadotropin (eCG) previously known as Pregnant Mare Serum Gonadotropin (PMSG) has been used for decades in regulating reproduction in various domestic animal species. Its use necessitates a good measurement of its bioactivity in commercial preparations. The EUROPEAN PHARMACOPEIA (EP 7.0) recommends 5-6 subcutaneous injections in immature female rats for the in vivo bioassay for eCG as in the case for measurement of FSH bioactivity in the Steelman & Pohley assay (1953). This recommendation is in marked contrast with the classical and long-time used PMSG assay of Cole & Erway (1941) that includes only one subcutaneous injection, 3 days before measurement of ovarian weight. As this difference introduces much confusion in the determination of eCG/PMSG bioactivity in commercial sources, we have performed parallel assays of several PMSG preparations, with both protocols. The single-injection protocol takes into account the long half-life of eCG in bloodstream and provokes an ovarian stimulation at lower concentrations than the multiple-injection protocol. As the single-injection protocol also mimicks the protocol used in cattle, it is preferable to the multiple injection protocol of the current EP.
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hal-02620640 , version 1 (26-10-2021)

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Yves Combarnous, Julie Mariot, Lauriane Relav, - Thi Mong Diep Nguyen, Danièle Klett. Choice of protocol for the in vivo bioassay of equine chorionic gonadotropin (eCG/PMSG) in immature female rats. Theriogenology, 2019, 130, pp.99-102. ⟨10.1016/j.theriogenology.2019.03.004⟩. ⟨hal-02620640⟩
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