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Choice of protocol for the in vivo bioassay of equine chorionic gonadotropin (eCG/PMSG) in immature female rats

Abstract : Equine Chorionic Gonadotropin (eCG) previously known as Pregnant Mare Serum Gonadotropin (PMSG) has been used for decades in regulating reproduction in various domestic animal species. Its use necessitates a good measurement of its bioactivity in commercial preparations. The EUROPEAN PHARMACOPEIA (EP 7.0) recommends 5-6 subcutaneous injections in immature female rats for the in vivo bioassay for eCG as in the case for measurement of FSH bioactivity in the Steelman & Pohley assay (1953). This recommendation is in marked contrast with the classical and long-time used PMSG assay of Cole & Erway (1941) that includes only one subcutaneous injection, 3 days before measurement of ovarian weight. As this difference introduces much confusion in the determination of eCG/PMSG bioactivity in commercial sources, we have performed parallel assays of several PMSG preparations, with both protocols. The single-injection protocol takes into account the long half-life of eCG in bloodstream and provokes an ovarian stimulation at lower concentrations than the multiple-injection protocol. As the single-injection protocol also mimicks the protocol used in cattle, it is preferable to the multiple injection protocol of the current EP.
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https://hal.inrae.fr/hal-02620640
Contributor : Migration Prodinra <>
Submitted on : Monday, May 25, 2020 - 11:37:07 PM
Last modification on : Monday, April 12, 2021 - 10:10:05 AM

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Yves Combarnous, Julie Mariot, Lauriane Relav, - Thi Mong Diep Nguyen, Danièle Klett. Choice of protocol for the in vivo bioassay of equine chorionic gonadotropin (eCG/PMSG) in immature female rats. Theriogenology, Elsevier, 2019, 130, pp.99-102. ⟨10.1016/j.theriogenology.2019.03.004⟩. ⟨hal-02620640⟩

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