A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance
Fanghao Wan
(1, 2)
,
Chuanlin Yin
(3)
,
Rui Tang
(2, 4)
,
Maohua Chen
(5)
,
Qiang Wu
(1)
,
Cong Huang
(1, 6)
,
Wanqiang Qian
(2)
,
Omar Rota-Stabelli
(7)
,
Nianwan Yang
(1)
,
Shuping Wang
(8)
,
Guirong Wang
(1)
,
Guifen Zhang
(1)
,
Jianyang Guo
(1)
,
Liuqi (aloy) Gu
(9)
,
Longfei Chen
(3)
,
Longsheng Xing
(2)
,
Yu Xi
(2)
,
Feiling Liu
(1)
,
Kejian Lin
(1)
,
Mengbo Guo
(1)
,
Wei Liu
(1)
,
Kang He
(3)
,
Ruizheng Tian
(5)
,
Emmanuelle Joly
(10)
,
Pierre Franck
(11)
,
Myriam Siegwart
(11)
,
Lino Ometto
(7, 12)
,
Gianfranco Anfora
(7, 13)
,
Mark Blaxter
(14)
,
Camille Meslin
(10)
,
Petr Nguyen
(15, 16)
,
Martina Dalíková
(15, 16)
,
František Marec
(15)
,
Jerome Olivares
(11)
,
Sandrine Maugin
(11)
,
Jianru Shen
(1)
,
Jinding Liu
(1)
,
Jinmeng Guo
(1)
,
Jiapeng Luo
(3)
,
Bo Liu
(2)
,
Wei Fan
(2)
,
Likai Feng
(17)
,
Xianxin Zhao
(3)
,
Xiong Peng
(5)
,
Kang Wang
(5)
,
Lang Liu
(5)
,
Haixia Zhan
(2)
,
Wanxue Liu
(1)
,
Guoliang Shi
(1, 18)
,
Chunyan Jiang
(1, 18)
,
Jisu Jin
(1, 6)
,
Xiaoqing Xian
(1)
,
Sha Lu
(1, 18)
,
Mingli Ye
(19)
,
Meizhen Li
(3)
,
Minglu Yang
(20)
,
Renci Xiong
(20)
,
James R. Walters
(9)
,
Fei Li
(3)
1
State Key Laboratory for Biology of Plant Diseases and Insect Pests
2 CAAS - Chinese Academy of Agricultural Sciences
3 Zhejiang University [Hangzhou, China]
4 State key laboratory of Integrated Management of pest Insects and Rodents, Institute of Zoology
5 Northwest A and F University
6 Hunan Agricultural University [Changsha]
7 FEM - Fondazione Edmund Mach - Edmund Mach Foundation [Italie]
8 Technical Centre for Animal Plant and Food Inspection and Quarantine
9 KU - University of Kansas [Lawrence]
10 iEES Paris - Institut d'écologie et des sciences de l'environnement de Paris
11 PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles
12 Department of Biology and Biotechnology
13 UNITN - Università degli Studi di Trento = University of Trento
14 Edin. - University of Edinburgh
15 BIOLOGY CENTRE CAS - Biology Centre of the Czech Academy of Sciences
16 Faculty of Science
17 Xinjiang Academy of Agricultural and Reclamation Sciences
18 Qingdao Agricultural University
19 Zhejiang Shuren University
20 Tarim University
2 CAAS - Chinese Academy of Agricultural Sciences
3 Zhejiang University [Hangzhou, China]
4 State key laboratory of Integrated Management of pest Insects and Rodents, Institute of Zoology
5 Northwest A and F University
6 Hunan Agricultural University [Changsha]
7 FEM - Fondazione Edmund Mach - Edmund Mach Foundation [Italie]
8 Technical Centre for Animal Plant and Food Inspection and Quarantine
9 KU - University of Kansas [Lawrence]
10 iEES Paris - Institut d'écologie et des sciences de l'environnement de Paris
11 PSH - Unité de recherche Plantes et Systèmes de Culture Horticoles
12 Department of Biology and Biotechnology
13 UNITN - Università degli Studi di Trento = University of Trento
14 Edin. - University of Edinburgh
15 BIOLOGY CENTRE CAS - Biology Centre of the Czech Academy of Sciences
16 Faculty of Science
17 Xinjiang Academy of Agricultural and Reclamation Sciences
18 Qingdao Agricultural University
19 Zhejiang Shuren University
20 Tarim University
Fanghao Wan
- Fonction : Auteur
- PersonId : 987332
Chuanlin Yin
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- ORCID : 0000-0003-0417-7703
Rui Tang
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- ORCID : 0000-0002-9313-0802
Qiang Wu
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- ORCID : 0000-0002-3005-4072
Cong Huang
- Fonction : Auteur
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- ORCID : 0000-0001-6149-4989
Wei Liu
- Fonction : Auteur
- PersonId : 756839
- ORCID : 0000-0003-0480-2097
Kang He
- Fonction : Auteur
- PersonId : 791005
- ORCID : 0000-0002-7270-8118
Emmanuelle Joly
- Fonction : Auteur
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- IdHAL : emmanuelle-joly
- ORCID : 0000-0002-6904-2036
- IdRef : 137606206
Pierre Franck
- Fonction : Auteur
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- IdHAL : pierre-franck
- ORCID : 0000-0002-1904-8325
- IdRef : 136706657
Myriam Siegwart
- Fonction : Auteur
- PersonId : 1204069
Lino Ometto
- Fonction : Auteur
- PersonId : 799438
- ORCID : 0000-0002-2679-625X
Camille Meslin
- Fonction : Auteur
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- IdHAL : camille-meslin
- ORCID : 0000-0003-2120-0936
- IdRef : 168568098
Petr Nguyen
- Fonction : Auteur
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- ORCID : 0000-0003-1395-4287
Martina Dalíková
- Fonction : Auteur
- PersonId : 800775
- ORCID : 0000-0002-0266-8894
František Marec
- Fonction : Auteur
- PersonId : 800776
- ORCID : 0000-0002-6745-5603
Jerome Olivares
- Fonction : Auteur
- PersonId : 1205180
Sandrine Maugin
- Fonction : Auteur
- PersonId : 1205299
Wei Fan
- Fonction : Auteur
- PersonId : 768748
- ORCID : 0000-0001-5036-8733
Fei Li
- Fonction : Auteur
- PersonId : 758846
- ORCID : 0000-0002-8410-5250
Résumé
The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.
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