Exfoliative toxin E, a new <em>Staphylococcus aureus</em> virulence factor with host-specific activity - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Scientific Reports Year : 2019

Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity

Ricardo Mariutti
  • Function : Author
Eric Guédon
Sergine Even
Raghuvir K. Arni
  • Function : Author
Yves Le Loir

Abstract

Exfoliative toxins (ETs) are secreted virulence factors produced by staphylococci. These serine proteases specifically cleave desmoglein 1 (Dsg1) in mammals and are key elements in staphylococcal skin infections. We recently identified a new et gene in S. aureus O46, a strain isolated from ovine mastitis. In the present study, we characterized the new et gene at a genetic level and the enzymatic activity of the deduced protein. The S. aureus O46 genome was re-assembled, annotated and compared with other publicly available S. aureus genomes. The deduced amino acid sequence of the new et gene shared 40%, 53% and 59% sequence identity to those of ETA, ETB and ETD, respectively. The new et gene shared the same genetic vicinity and was similar in other S. aureus strains bearing this gene. The recombinant enzyme of the new et gene caused skin exfoliation in vivo in neonatal mice. The new et-gene was thus named ete, encoding a new type (type E) of exfoliative toxin. We showed that ETE degraded the extracellular segments of Dsg1 in murine, ovine and caprine epidermis, as well as in ovine teat canal epithelia, but not that in bovine epidermis. We further showed that it directly hydrolyzed human and swine Dsg1 as well as murine Dsg1α and Dsg1β, but not canine Dsg1 or murine Dsg1γ. Molecular modeling revealed a correlation between the preferred orientation of ETE docking on its Dsg1 cleavage site and species-specific cleavage activity, suggesting that the docking step preceding cleavage accounts for the ETE species-specificity. This new virulence factor may contribute to the bacterial colonization on the stratified epithelia in certain ruminants with mastitis.
Fichier principal
Vignette du fichier
s41598-019-52777-3_1.pdf (2.47 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02625122 , version 1 (26-05-2020)

Licence

Identifiers

Cite

Ichiro Imanishi, Aurélie Nicolas, Ana-Carolina Barbosa Caetano, Thiago Luiz de Paula Castro, Natayme Rocha Tartaglia, et al.. Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity. Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-52777-3⟩. ⟨hal-02625122⟩
48 View
62 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More