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Article dans une revue

Use of GBS markers to distinguish among lucerne varieties, with comparison to morphological traits

Abstract : Variety registration of lucerne often fails because of the difficulty to pass the distinction test. This test is presently based on phenotypic traits. We proposed to evaluate if high-throughput genotyping of varieties with genotyping-by-sequencing markers could help to distinguish among 20 varieties and if the genetic distances were correlated to phenotypic ones. Genotyping was performed either on 40 individuals or on 4 bulks of 100 plants per variety. At the methodological level, we obtained a high number of polymorphic markers, a precise determination of the allele dosage on the individuals, a high reproducibility of allele frequency estimated on bulks and a good correlation between allele frequencies scored on individuals or on bulks. Each pair of the 20 varieties was significantly distinct when using the markers. In addition, the varieties grouped in a way that was consistent with their genetic origin. Genetic distances were correlated to phenotypic distances obtained from data collected in the distinction tests. We suggest that molecular markers could be useful to assist distinction tests during the official trials for registration of lucerne varieties.
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Article dans une revue
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https://hal.inrae.fr/hal-02626116
Déposant : Migration Prodinra <>
Soumis le : mardi 26 mai 2020 - 16:23:10
Dernière modification le : lundi 2 novembre 2020 - 09:22:02

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Bernadette Julier, Philippe Barre, Paola Lambroni, Sabrina Delaunay, Muriel Thomasset, et al.. Use of GBS markers to distinguish among lucerne varieties, with comparison to morphological traits. Molecular Breeding, Springer Verlag, 2018, 38 (11), pp.1-12. ⟨10.1007/s11032-018-0891-1⟩. ⟨hal-02626116⟩

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