Skip to Main content Skip to Navigation
Journal articles

Commensal gut bacteria modulate phosphorylation-dependent PPAR gamma transcriptional activity in human intestinal epithelial cells

Abstract : In healthy subjects, the intestinal microbiota interacts with the host's epithelium, regulating gene expression to the benefit of both, host and microbiota. The underlying mechanisms remain poorly understood, however. Although many gut bacteria are not yet cultured, constantly growing culture collections have been established. We selected 57 representative commensal bacterial strains to study bacteria-host interactions, focusing on PPAR gamma, a key nuclear receptor in colonocytes linking metabolism and inflammation to the microbiota. Conditioned media (CM) were harvested from anaerobic cultures and assessed for their ability to modulate PPAR gamma using a reporter cell line. Activation of PPAR gamma transcriptional activity was linked to the presence of butyrate and propionate, two of the main metabolites of intestinal bacteria. Interestingly, some stimulatory CMs were devoid of these metabolites. A Prevotella and an Atopobium strain were chosen for further study, and shown to upregulate two PPAR gamma-target genes, ANGPTL4 and ADRP. The molecular mechanisms of these activations involved the phosphorylation of PPAR gamma through ERK1/2. The responsible metabolites were shown to be heat sensitive but markedly diverged in size, emphasizing the diversity of bioactive compounds found in the intestine. Here we describe different mechanisms by which single intestinal bacteria can directly impact their host's health through transcriptional regulation.
Document type :
Journal articles
Complete list of metadata

Cited literature [52 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02628360
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Tuesday, May 26, 2020 - 10:52:50 PM
Last modification on : Thursday, October 14, 2021 - 9:16:30 AM

File

2017_Nepelska_Scientific Repor...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Malgorzata Nepelska, Tomas de Wouters, Elsa Jacouton, Fabienne Beguet-Crespel, Nicolas Lapaque, et al.. Commensal gut bacteria modulate phosphorylation-dependent PPAR gamma transcriptional activity in human intestinal epithelial cells. Scientific Reports, Nature Publishing Group, 2017, 7, ⟨10.1038/srep43199⟩. ⟨hal-02628360⟩

Share

Metrics

Record views

49

Files downloads

66