Centrifugal ultrafiltration of genipap fruit extracts after mechanical/sonic enzymatic treatment was performed through 50,30,10 and 3kDa molecula rweight cutoff (MWCO) (15min;10 °C; 9425g). Volume, volume retention factor (VRF), flux, genipin and glucose concentrations were determined. Separation efficiency, retention ,transmission coefficients (RC,TC), and membrane selectivity were estimated. Reduction in MWCO increased volume in the retentate(7.6 + 0.0 mL),decreased VRF (1.9 +0.0) and flux (0.06+0.00mL.cm 2. min 1) after3kDa ultrafiltration. Genipin and glucose reached equilibrium in retentate and permeate (17+3.3; 16+1.5mg.mL 1; 41+2.2 and 41+4.8 mg.mL 1) after 3kDa ultrafiltration. The highest removal of genipin was 0.57[+]0.1.RC and TC were 0.5+0.1; selectivity for genipin was 0.970 +.0 after 3kDa separation.