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Article dans une revue

Meiotic recombinaison analyses of individual chromosomes in male domestic pigs (sus scrofa domestica)

Abstract : For the first time in the domestic pig, meiotic recombination along the 18 porcine autosomes was directly studied by immunolocalization of MLH1 protein. In total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 13,969 recombination sites were mapped. Individual chromosomes for 113 of the 436 cells (representing 2,034 synaptonemal complexes) were identified by immunostaining and fluorescence in situ hybridization (FISH). The average total length of autosomal synaptonemal complexes per cell was 190.3 µm, with 32.0 recombination sites (crossovers), on average, per cell. The number of crossovers and the lengths of the autosomal synaptonemal complexes showed significant intra- (i.e. between cells) and inter-individual variations. The distributions of recombination sites within each chromosomal category were similar: crossovers in metacentric and submetacentric chromosomes were concentrated in the telomeric regions of the p- and q-arms, whereas two hotspots were located near the centromere and in the telomeric region of acrocentrics. Lack of MLH1 foci was mainly observed in the smaller chromosomes, particularly chromosome 18 (SSC18) and the sex chromosomes. All autosomes displayed positive interference, with a large variability between the chromosomes.
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Soumis le : mercredi 27 mai 2020 - 16:26:14
Dernière modification le : mardi 2 mars 2021 - 09:22:03

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Nicolas Mary, Harmonie Barasc, Stephane Ferchaud, Yvon Billon, Frederic Meslier, et al.. Meiotic recombinaison analyses of individual chromosomes in male domestic pigs (sus scrofa domestica). PLoS ONE, Public Library of Science, 2014, 9 (6), pp.Non Paginé. ⟨10.1371/journal.pone.0099123⟩. ⟨hal-02634784⟩

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