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Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.

Abstract : To assess the influence of the environment on fruit metabolism, Tomato (Solanum lycopersicum) cv. Moneymaker plants were grown under contrasting conditions (optimal for commercial production, water limitation or shading) and locations. Samples were harvested at nine stages of development and 36 enzymes activities of central metabolism were measured, as well as protein and major metabolites such as hexoses, sucrose, starch, organic acids and amino acids. The most remarkable result was the high reproducibility of enzyme time-courses throughout development irrespective of conditions or location. Hierarchical clustering of enzyme activities also revealed tight relationships between metabolic pathways and phases of development. Thus, cell division was characterized by high hexokinases, pyruvate kinase and TCA-cycle enzymes, indicating ATP-production as a priority, whereas cell expansion was characterized by enzymes involved in the lower part of glycolysis, suggesting a metabolic reprogramming towards anapleurosis. As expected enzymes involved in the accumulation of sugars, citrate and glutamate were strongly increased at ripening. However, a group of enzymes involved in ATP-production, which is probably fueled by starch degradation, were also increased. Metabolites appeared more sensitive than enzymes to the environment, although such differences tended to decrease at ripening. The integration of enzyme and metabolite data obtained under contrasting growth conditions using principal component analysis suggests that at the exception of alanine amino-transferase and glutamate, as well as malate dehydrogenase and malate, there are no links between single enzyme activities and metabolite fluxes or levels.
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https://hal.inrae.fr/hal-02639362
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Submitted on : Thursday, May 28, 2020 - 11:53:30 AM
Last modification on : Monday, May 10, 2021 - 2:57:45 PM

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Benoit Biais, Camille Bénard, Bertrand Beauvoit, Sophie Colombie, Duyên Prodhomme, et al.. Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.. Plant Physiology, American Society of Plant Biologists, 2014, 164, pp.1204-1221. ⟨10.1104/pp.113.231241⟩. ⟨hal-02639362⟩

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