OhrRA functions as a redox-responsive system controlling toxinogenesis in Bacillus cereus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Proteomics Year : 2013

OhrRA functions as a redox-responsive system controlling toxinogenesis in Bacillus cereus

Abstract

Bacillus cereus OhrR is a member of the subgroup of the MarR (multiple antibiotic resistance) family of transcriptional regulators that use a cysteine-based redox sensing mechanism. OhrA is a thiol-dependent, peroxidase-like protein. The dual OhrRA system triggers B. cereus adaptation in response to redox changes, such as those encountered in the environment of the gastrointestinal tract. Here, we investigated the role of OhrRA in toxinogenesis. Comparative shotgun analysis of exoproteomes from ΔohrA, ΔohrR and wild-type cells revealed significant changes in the abundance levels of toxin-related proteins depending on the extracellular redox potential.We complemented these data by measuring the DNA binding activity of reduced and oxidized recombinant OhrR on toxin and putative toxin promoter regions. Furthermore, transcriptomic data and OhrRA-dependent, antiproliferative activity of the B. cereus exoproteome on Caco-2 human epithelial cells were recorded. The results indicate that OhrR controlled toxin gene expression directly or indirectly in a redox- and toxin-dependent manner, and may function as a repressor or an activator. Moreover, we found that OhrR restricts toxindependent antiproliferative activity of the B. cereus exoproteome whatever the growth conditions, while the restrictive impact of OhrA occurs only under low ORP anoxic conditions.
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Dates and versions

hal-02642402 , version 1 (28-05-2020)

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Gérémy Clair, Alain Lhorphelin, J. Armengaud, Catherine Duport. OhrRA functions as a redox-responsive system controlling toxinogenesis in Bacillus cereus. Journal of Proteomics, 2013, 94, pp.527-539. ⟨10.1016/j.prot.2013.10.024⟩. ⟨hal-02642402⟩
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