Flavanone metabolites decrease monocyte adhesion to TNF-alpha-activated endothelial cells by modulating expression of atherosclerosis-related genes - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles British Journal of Nutrition Year : 2013

Flavanone metabolites decrease monocyte adhesion to TNF-alpha-activated endothelial cells by modulating expression of atherosclerosis-related genes

Abstract

Flavanones are found specifically and abundantly in citrus fruits. Their beneficial effect on vascular function is well documented. However, little is known about their cellular and molecular mechanisms of action in vascular cells. The goal of the present study was to identify the impact of flavanone metabolites on endothelial cells and decipher the underlying molecular mechanisms of action. We investigated the impact of naringenin and hesperetin metabolites at 0.5, 2 and 10 mu M on monocyte adhesion to TNF-alpha-activated human umbilical vein endothelial cells (HUVEC) and on gene expression. Except hesperetin-7-glucuronide and naringenin-7-glucuronide (N7G), when present at 2mM, flavanone metabolites (hesperetin-3'-sulphate, hesperetin-3'-glucuronide and naringenin-4'-glucuronide (N4'G)) significantly attenuated monocyte adhesion to TNF-alpha-activated HUVEC. Exposure of both monocytes and HUVEC to N4'G and N7G at 2 mu M resulted in a higher inhibitory effect on monocyte adhesion. Gene expression analysis, using TaqMan Low-Density Array, revealed that flavanone metabolites modulated the expression of genes involved in atherogenesis, such as those involved in inflammation, cell adhesion and cytoskeletal organisation. In conclusion, physiologically relevant concentrations of flavanone metabolites reduce monocyte adhesion to TNF-alpha-stimulated endothelial cells by affecting the expression of related genes. This provides a potential explanation for the vasculoprotective effects of flavanones
Fichier principal
Vignette du fichier
Chanet et al BJN 2013_1.pdf (384.34 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

hal-02642629 , version 1 (28-05-2020)

Identifiers

  • HAL Id : hal-02642629 , version 1
  • PRODINRA : 206755
  • PUBMED : 23332102
  • WOS : 000322784000001

Cite

Audrey Chanet, Dragan Milenkovic, Sylvain Claude, Jeanette A. M. Maier, Muhammad Kamran Khan, et al.. Flavanone metabolites decrease monocyte adhesion to TNF-alpha-activated endothelial cells by modulating expression of atherosclerosis-related genes. British Journal of Nutrition, 2013, 110 (4), pp.587-598. ⟨hal-02642629⟩
23 View
24 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More