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Dietary n-3 PUFA affect lipid metabolism and tissue function-related genes in bovine muscle

Abstract : Gene expression profiles of bovine longissimus muscle as affected by dietary n-3 v. n-6 fatty acid (FA) intervention were analysed by microarray pre-screening of >3000 muscle biology/meat quality-related genes as well as subsequent quantitative RT-PCR gene expression validation of genes encoding lipogenesis-related transcription factors (CCAAT/enhancer-binding protein beta, sterol regulatory element-binding transcription factor 1), key-lipogenic enzymes (acetyl-CoA carboxylase alpha (ACACA), fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD)), lipid storage-associated proteins (adipose differentiation-related protein (ADFP)) and muscle biology-related proteins (cholinergic receptor, nicotinic, alpha 1, farnesyl diphosphate farnesyl transferase 1, sema domain 3C (SEMA3C)). Down-regulation of ACACA (P=0.00), FASN (P=0.09) and SCD (P=0.02) gene expression upon an n-3 FA intervention directly corresponded to reduced SFA, MUFA and total FA concentrations in longissimus muscle, whereas changes in ADFP (P=0.00) and SEMA3C (P=0.05) gene expression indicated improved muscle function via enhanced energy metabolism, vasculogenesis, innervation and mediator synthesis. The present study highlights the significance of dietary n-3 FA intervention on muscle development, maintenance and function, which are relevant for meat quality tailoring of bovine tissues and modulating animal production-relevant physiological processes.
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Submitted on : Friday, May 29, 2020 - 4:02:17 AM
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Beate Hiller, Jean-François Hocquette, Isabelle Cassar-Malek, Gerd Nuernberg, Karin Nuernberg. Dietary n-3 PUFA affect lipid metabolism and tissue function-related genes in bovine muscle. British Journal of Nutrition, Cambridge University Press (CUP), 2012, 108 (5), pp.858-863. ⟨10.1017/S0007114511006179⟩. ⟨hal-02646135⟩



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