Conception rate and the calving interval of beef cows are known to be influenced by body reserves at calving and subsequent postpartum changes. However, few studies have focused on the effect of body reserve dynamics on both postpartum cyclicity and estrus expression. Two successive similar experiments (Year 1: n = 14; Year 2: n = 16) were carried out on primiparous Charolais cows reared indoors during winter to quantify the effects of adipose cell diameter at calving (ACDca) and their postpartum changes (ACDch) on cyclicity and estrus behavior. Cows were managed to calve with a body condition score (BCS, scale 0-5) of 2.5 (Year I) and 1.5 (Year 2). After calving cows were assigned to a Low vs. a High energy level diet until turn out to pasture in May. Within years ACDca was similar between Low and High groups whereas calving to turnout changes of body weight (BW), BCS and adipose cell diameter differed (P < 0.0001). The interval between calving and resumption of luteal activity was negatively correlated with ACDca (P = 0.001). Estrus duration (interval between first and last standing to be mounted (STBM)) was longer in Low than in High groups (P = 0.02). Number of STMB was higher in Low than in High cows. Adipose cell diameter at calving and postpartum changes had distinct effects on the components of reproductive performance; emphasizing the need to consider both amounts and changes of body lipids to predict relationships between nutrition and reproduction in cows.