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Lipid-deprived diet perturbs O-glycosylation of secretory proteins in rat mammary epithelial cells

Abstract : Nutrition modulates both production and composition of milk. Milk composition was studied in rats chronically fed a diet without additional lipids, and therefore eating only traces of the recommended supply of essential polyunsaturated fatty acid. Despite a large decrease in milk-protein synthesis, only protein composition, but not protein concentration, was found to change in the milk of rats following a lipid-deprived diet. Correlatively, we observed a substantial increase in the lactose concentration of milk. Analysis of milk proteins by two-dimensional electrophoresis demonstrated that the relative proportion of the various molecular forms of k-casein, an O-glycosylated protein, was modified in the milk of rats receiving the lipiddeprived diet. In tissues, differences in the two-dimensional pattern of k-casein between control and lipid-deprived rats were similar, if not identical. In contrast to k-casein, the molecular forms of a-lactalbumin, an N-glycosylated protein, were not affected by the diet. These data provide evidence that O-glycosylation of milk proteins in the secretory pathway of mammary epithelial cells is modulated by the lipid content of experimental diets.
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Submitted on : Saturday, May 30, 2020 - 11:33:18 PM
Last modification on : Friday, August 20, 2021 - 1:56:01 PM

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Françoise Lavialle, Eric Chanat. Lipid-deprived diet perturbs O-glycosylation of secretory proteins in rat mammary epithelial cells. animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2008, 2 (4), pp.491-499. ⟨10.1017/S1751731107001309⟩. ⟨hal-02662151⟩

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