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Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells

Abstract : In the present study, we investigated the ability of human fetal membranes (amnion and choriodecidua) to regulate human maternal uterine cell functions through the secretion of surfactant protein (SP)-A and SP-D at the end of pregnancy. We detected the expression of both SP-A (SP-A1 and SP-A2) and SP-D by quantitative reverse transcription polymerase chain reaction. Immunohistochemistry revealed that human fetal membranes expressed both SP-A and SP-D. By Western blot analysis, we demonstrated that SP-A protein expression was predominant in choriodecidua, whereas the amnion predominantly expressed SP-D. Only the secretion of SP-A was evidenced in the culture supernatants of amnion and choriodecidua explants by immunodot blot and confirmed by Western blot. Exogenous human purified SP-A induced stress fiber formation in cultured human myometrial cells via a pathway involving Rho-kinase. Conditioned medium from choriodecidua and amnion explants mimicked the SP-A effect. Treatment of myometrial cells with SP-A-depleted conditioned medium from choriodecidua or amnion explants failed to change the actin dynamic. These data indicate that SP-A released by human fetal membranes is able to exert a paracrine regulation of F-actin filament organization in myometrial cells.
Keywords : myometrium pregnancy
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https://hal.inrae.fr/hal-02662250
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Submitted on : Saturday, May 30, 2020 - 11:46:23 PM
Last modification on : Thursday, August 5, 2021 - 3:53:36 AM

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Michelle Breuiller-Fouché, Olivier Dubois, Mourad Sediki, Ignacio Garcio-Verdugo, Nades Palaniyar, et al.. Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells. AJP - Endocrinology and Metabolism, American Physiological Society, 2010, 298 (6), pp.E1188-E1197. ⟨10.1152/ajpendo.00746.2009⟩. ⟨hal-02662250⟩

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