Inhibition of myoblast differentiation by Sfrp1 and Sfrp2 - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Cell and Tissue Research Year : 2008

Inhibition of myoblast differentiation by Sfrp1 and Sfrp2

Abstract

Secreted Frizzled-related proteins (Sfrps) are extracellular regulators of Wnt signalling and play important roles in developmental and oncogenic processes. They are known to be upregulated in regenerating muscle and in myoblast cultures but their function is unknown. Here, we show that the addition of recombinant Sfrp1 or Sfrp2 to C2C12 cell line cultures or to primary cultures of satellite cells results in the inhibition of myotube formation with no significant effect on the cell cycle or apoptosis. Even though at confluence, treated and untreated cultures are identical in appearance, analyses have shown that, for maximum effect, the cells have to be treated while they are proliferating. Furthermore, removal of Sfrp from the culture medium during differentiation restores normal myotube formation. We conclude that Sfrp1 and Sfrp2 act to prevent myoblasts from entering the terminal differentiation process.

Dates and versions

hal-02662612 , version 1 (31-05-2020)

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Simon Descamps, Hayat Arzouk, Francis Bacou, Henri Bernardi, Yann Fédon, et al.. Inhibition of myoblast differentiation by Sfrp1 and Sfrp2. Cell and Tissue Research, 2008, 332, pp.299-306. ⟨10.1007/s00441-008-0574-z⟩. ⟨hal-02662612⟩
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