HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Epididymis-specific promoter-driven gene targeting: a transcription factor which regulates epididymis-specific gene expression

Abstract : Mammalian spermatozoa undergo several modification and finally acquire the ability to fertilize during epididymal transit. One of the distinct features of the epididymis is that it displays a highly regionalized pattern of gene expression. This tissue-, region-, and cell-specific pattern of gene expression is critical for the maintenance of a fully functional epididymis. One would hypothesize that disrupting this process provides an ideal approach to male contraception, since it would not interfere with testicular endocrine output or sperm production. To achieve this purpose, we studied a cluster of epididymis-specific lipocalin genes for understanding the specific mechanisms involved in the control of gene expression in the epididymis. We have identified six epididymis-specific lipocalin genes that are differently regulated and regionalized in the epididymis. Lipocalin 5 [Lcn5 or epididymal retinoic acid-binding protein (E-RABP)] is a member of this epididymis-specific lipocalin gene cluster, which binds hydrophobic molecules such as retinoic acid. We have previously shown that the 5 kb promoter fragment of the Lcn5 gene confers both androgen-dependent regulation and epididymis-specific gene expression in transgenic mice whereas 0.6 kb promoter fragment does not. To further narrow down the important cis-regulatory elements that regulate gene expression in the epididymis, we studied the Lcn5 promoter in both transgenic mice and immortalized epididymal cells. We have found that 1.8 kb promoter fragment of the Lcn5 gene was sufficient for tissue- and region-specific expression in transgenic mice, and that a transcription factor Forkhead box A2 (Foxa2) interacts with the androgen receptor and binds to the 100 bp fragment of the Lcn5 promoter between 1.2 and 1.3 kb. Our finding provides a framework for further analysis of the epididymal lipocalin gene regulation and modulated control of epididymis-specific expression.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02662893
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 1:30:58 AM
Last modification on : Thursday, March 31, 2022 - 3:42:17 AM

Links full text

Identifiers

Collections

Citation

Kichiya Suzuki, Yu Xiuping, Pierre Chaurand, Yoshihiko Araki, Jean-Jacques Lareyre, et al.. Epididymis-specific promoter-driven gene targeting: a transcription factor which regulates epididymis-specific gene expression. Molecular and Cellular Endocrinology, Elsevier, 2006, 250, pp.184-189. ⟨10.1016/j.mce.2005.12.043⟩. ⟨hal-02662893⟩

Share

Metrics

Record views

7