Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Food Chemistry Année : 2009

Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds

Résumé

In order to fully explore the biofunctional potential of proanthocyanidins (PA), isolated and well-characterised PA dimers are of great importance. Current methods to obtain pure A- and B-type dimers are laborious, because they comprise multiple chromatographic steps, often yielding only one or two specific dimers. In the current study, an efficient isolation procedure is described, to isolate a large variety of A-type dimers from peanut skins and B-type dimers from grape seeds. Yields increased 20-400 times for A-type dimers and about 10 times for B-type dimers compared to other methods with a lesser number of chromatographic steps. Dinners isolated from peanut skins were identified as; epicatechin-(2-O-7, 4-8)-catechin (A1), epicatechin-(2-O-7, 4-8)-epicatechin (A2), epicatechin-(2-O-7, 4-6)-catechin, epicatechin-(2-O-7, 4-8)-entcatechin, isolated from peanut skins for the first time, and epicatechin-(4-6)-catechin (B7). Dimers from grape seeds were identified as; epicatechin-(4-8)-catechin (B1), epicatechin-(4-8)-epicatechin (132), catechin-(4-8)-catechin (B3) and catechin-(4-8)-epicatechin (B4)

Dates et versions

hal-02663689 , version 1 (31-05-2020)

Identifiants

Citer

Maaike M. Appeldoorn, Mark Sanders, Jean-Paul Vincken, Veronique V. Cheynier, Christine Le Guerneve, et al.. Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds. Food Chemistry, 2009, 117 (4), pp.713-720. ⟨10.1016/j.foodchem.2009.04.047⟩. ⟨hal-02663689⟩
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