Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics

Abstract : Muscle contractile and metabolic characteristics were studied on nine cloned and eight non-cloned (control) heifers. The animals were submitted to repeated biopsies of the semitendinosus (ST) muscle at the ages of 8, 12, 18 and 24 months. The contractile type was determined from the proportion of the different myosin heavy chain (MyHC) isoforms separated by electrophoresis. Glycolytic metabolism was assessed by lactate dehydrogenase (LDH) activity, and oxidative metabolism was assessed by isocitrate dehydrogenase (ICDH), cytochrome-c oxidase (COX) and b-hydroxyacyl-CoA dehydrogenase (HAD) activities. In cloned heifers at 8 months of age, there was a greater proportion of MyHC I (slow oxidative isoform) and MyHC IIa (fast oxido-glycolytic isoform), a lower proportion of MyHC IIx (fast glycolytic isoform), greater COX and HAD activity and a lower LDH/ICDH ratio compared with control heifers. Thus, young cloned heifers had slower muscle types associated with a more oxidative muscular metabolism than control heifers. From 12 months of age onwards, no significant differences were observed between cloned and control heifers. A delay in muscle differentiation and maturation in cloned heifers is hypothesised and discussed.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.inrae.fr/hal-02665080
Déposant : Migration Prodinra <>
Soumis le : dimanche 31 mai 2020 - 06:03:59
Dernière modification le : vendredi 19 février 2021 - 09:12:46

Lien texte intégral

Identifiants

Collections

Citation

Catherine Jurie, Brigitte Picard, Yvan Heyman, Isabelle Cassar-Malek, Pascale Chavatte Palmer, et al.. Comparison of cloned and non-cloned Holstein heifers in muscle contractile and metabolic characteristics. animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2009, 3 (2), pp.244-250. ⟨10.1017/S1751731108003406⟩. ⟨hal-02665080⟩

Partager

Métriques

Consultations de la notice

7