Skip to Main content Skip to Navigation
Journal articles

The involvement of beta-1,4-galactosyltransferase and N-acetylglucosamine residues in fertilization has been lost in the horse

Abstract : Background: In human and rodents, sperm-zona pellucida binding is mediated by a sperm surface Galactosyltransferase that recognizes N-Acetylglucosamine residues on a glycoprotein ZPC. In large domestic mammals, the role of these molecules remains unclear: in bovine, they are involved in sperm-zona pellucida binding, whereas in porcine, they are not necessary. Our aim was to clarify the role of Galactosyltransferase and N-Acetylglucosamine residues in sperm-zona pellucida binding in ungulates. For this purpose, we analyzed the mechanism of sperm-zona pellucida interaction in a third ungulate: the horse, since the Galactosyltransferase and N-Acetylglucosamine residues have been localized on equine gametes. Methods: We masked the Galactosyltransferase and N-Acetylglucosamine residues before the coincubation of gametes. Galactosyltransferase was masked either with an anti-Galactosyltransferase antibody or with the enzyme substrate, UDP Galactose. N-Acetylglucosamine residues were masked either with a purified Galactosyltransferase or with an anti-ZPC antibody. Results and discussion: The number of spermatozoa bound to the zona pellucida did not decrease after the masking of Galactosyltransferase or N-Acetylglucosamine. So, these two molecules may not be necessary in the mechanism of in vitro sperm-zona pellucida interaction in the horse. Conclusion: The involvement of Galactosyltransferase and N-Acetylglucosamine residues in sperm-zona pellucida binding may have been lost during evolution in some ungulates, such as porcine and equine species.
Keywords : N-ACETYLGLUCOSAMINE
Document type :
Journal articles
Complete list of metadata

Cited literature [39 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02665172
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 6:15:20 AM
Last modification on : Thursday, August 5, 2021 - 3:49:02 AM

File

28421_20090423085649123_1.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Sylvie Mugnier, Stéphane Boittin, Cécile Douet, Philippe Monget, Michèle Magistrini, et al.. The involvement of beta-1,4-galactosyltransferase and N-acetylglucosamine residues in fertilization has been lost in the horse. Reproductive Biology and Endocrinology, BioMed Central, 2008, 6:51, pp.1-9. ⟨10.1186/1477-7827-6-51⟩. ⟨hal-02665172⟩

Share

Metrics

Record views

54

Files downloads

113