Skip to Main content Skip to Navigation
Journal articles

Cryoconservation des embryons des espèces domestiques

Abstract : Cryopreservation of mammalian embryos was developed during the late 1970s. Since then, the cryopreservation techniques have been diversified and improved to try to cryopreserve embryos from most species, at different developmental stages, and more sensitive embryos such as in vitro produced, cloned or biopsied embryos. Slow freezing (or equilibrium freezing) and vitrification are the main cryopreservation techniques commonly used. Slow freezing attempts to maintain a delicate balance between cryoprotectants and the aqueous embryo compartment, whereas the strategy of the vitrification method is a rapid solidification of liquid without ice crystal formation. The OPS technique (Open Pulled Straw) is a faster vitrification process. All techniques offer advantages and disadvantages, but slow freezing is currently the most used on farm applications in sheep, goats and cattle with embryos produced in vivo. In pigs, only the OPS technique allows to cryopreserve embryos with good success. In the equine, none of these different techniques gives good results : only a few foals have been born after transfer of cryopreserved embryos.
Mots-clés : BIOTECHNOLOGIE
Document type :
Journal articles
Complete list of metadata

Cited literature [87 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02675494
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Sunday, May 31, 2020 - 5:41:08 PM
Last modification on : Thursday, August 5, 2021 - 3:42:31 AM

File

Prod_Anim_2005_18_1_02_1.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Florence Guignot. Cryoconservation des embryons des espèces domestiques. Productions animales, Institut National de la Recherche Agronomique, 2005, 18 (1), pp.27-35. ⟨hal-02675494⟩

Share

Metrics

Record views

115

Files downloads

95