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Characterization of pigments from different high speed countercurrent chromatography wine fractions

Abstract : A red wine, made from Cabernet Sauvignon (60%) and Tannat (40%) cultivars, was fractionated by high speed countercurrent chromatography (HSCCC). The biphasic solvent system consisting of tertbutyl methyl ether/n-butanol/acetonitrile/water (2/2/1/5, acidified with 0.1% trifluoroacetic acid) was chosen for its demonstrated efficiency in separating anthocyanins. The different native and derived anthocyanins were identified on the basis of their UV-visible spectra, their elution time on reversed-phase high-performance liquid chromatography (HPLC), and their mass spectra, before and after thiolysis. The HSCCC method allowed the separation of different families of anthocyanin-derived pigments that were eluted in different fractions according to their structures. The hydrosoluble fraction was almost devoid of native anthocyanins. Further characterization (glucose quantification, UV-visible absorbance measurements) indicated that it contained flavanol and anthocyanin copolymers in which parts of the anthocyanin units were in colorless forms. Pigments in the hydrosoluble fraction showed increased resistance to sulfite bleaching and to the nucleophilic attack of water.
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https://hal.inrae.fr/hal-02677518
Déposant : Migration Prodinra <>
Soumis le : dimanche 31 mai 2020 - 19:29:19
Dernière modification le : jeudi 12 novembre 2020 - 10:06:06

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Erika Salas, Montserrat Dueñas, Michael Schwarz, Peter Winterhalter, Veronique Cheynier, et al.. Characterization of pigments from different high speed countercurrent chromatography wine fractions. Journal of Agricultural and Food Chemistry, American Chemical Society, 2005, 53 (11), pp.4536-4546. ⟨10.1021/jf0478096⟩. ⟨hal-02677518⟩

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