This paper explores the potential of Actor Network Theory (ANT) in understanding how the process of interaction and translation between human and non-human actors contribute to the development, adoption and diffusion of science-based innovations linked to the transition to organic farming. The study relies on two case studies, the French Camargue case covering a range of technical and social innovations, and the case from Bulgaria focusing on the development of a technical and product innovation, i.e. a veterinary product for organic beekeeping. The paper shows the limitations of classical approaches in studying innovations since they underestimate the role of heterogeneous actors, their status, and how they interact with each other. We argue that focusing on actors’ interactions helps to better understand the so-called “uncertainties” and “turning points” in the innovation development, as well as to interpret them as natural elements. Moreover we argue that challenges to tackle should be problematized to increase the success of research programs. We also stress the importance of opinion leaders during the implementation and diffusion phase of the innovation.