The availability of complete genome sequences of numerous plant species has led to the study of various transcription factor families. These insights at the whole genome level can provide evolutionary clues for expansion of gene families and can help to predict their functions. DOF proteins are plantspecific transcription factors which have been implicated in diverse plant processes such as seed germination, seed development, carbohydrate and secondary metabolism, light mediated gene regulation, vascular tissue development, disease response, cell cycle regulation and hormone homeostasis. In this study, a comparative analysis of DOF TF family was carried out in Oryza sativa, Sorghum bicolor, Zea mays, Arabidopsis thaliana, Cajanus cajan, Cicer arietinum, Glycine max, Medicago truncatula, Gossypium raimondii and Gossypium arboretum. From the inferred phylogeny, it was observed that DOF family has been expanded with evolutionary process from singlecelled green algae Chlamydomonas reinhardtii having single DOF gene to tetraploid soybean with 78 DOF encoding genes. It was also inferred that DOF TFs regulating the seed development process in rice, sorghum and maize were evolved in Polaes-specific lineage. Investigations of gene structure has revealed that the several introns were lost from CrDof during evolution, so majority of DOF genes in higher plants are either intronless or have single intron. Some intron regain events have also been observed. Segmental as well as tandem duplications seemed to be responsible for expansion of DOF family in studied Poales. Whereas tandem duplication events seemed to be main route for the expansion of DOF family in Fabales. Gene duplication studies in Malvales suggested that largescale segmental duplications might have been solely responsible for the DOF family expansion in Gossypium species. These findings led us to hypothesize that most of the recent expansions of the DOF family have involved developmentrelated genes.