The objective of the current study was to identify biomarkers of branched chain amino acids (BCAA) intake status that could be linked to the best animal growth perfonnance. Three dose-response studics were carried out to collect blood and urine samples from pigs fed diets with increasing levels of isoleucine (Ile), valine (Val), or leucine (Leu) followed by a non-targeted LC-MS approach to characterize the metabolic profile of plasma and urine, when the level of BCAA in the diet is optimum for the best animal growth performance. The LC-MS method could separate and identify the plasma and urine metabolites which were discriminating the pigs fed optimum dietary level of Ile, Val, and Leu. Among the discriminating plasma and urine metabolites, that correlated closely to the highest animal growth performance, were plasma glycocholic acid and taurocholic acid as biomarkers of the optimum Ile level in the diet, and plasma creatine and urinary 2-aminoadipic acid, ascorbic acid, and choline as biomarkers of optimum Leu level. No good biomarkers were identified in the Val study.