In the context of protected designation of origin (PDO) wine production, wine organoleptic quality, and hence grape quality is a key target of vineyard management. LCA-based Improvement of Technical Management Routes (TMR) by choice of more environmentally friendly techniques needs to take into account this quality dimension in addition to the yield function usually considered in wine and grape LCAs. The aim of the paper is to present and discuss two proposals for including multi-criteria quality into eco-efficiency assessment of PDO grape production in order to support the choice, design and assessment of vineyard TMRs. We porpose the design of a combined quality index Q relating an observed multi-criteria quality to a atrgeted quality. A set of logical rules of inference (if...then...) was used considering several levels of correspondence of the product to the quality target. From this Index, a Quality Functional Unit (QFU =Q) and a Mass x Quality Functional Unit (MQFU=yield x Q) were defined. For application of Q to grape, a typology of grape quality targets with the different quality criteria and their levels for each grape type was established with expert-oenologists as a basis for Q. We tested the sensitivity of the two indexes to a change of quality target. We implemented the two FUs in LCA calculations on five al viticultural TMRs representing the Middle Loire Valley diversity for production of a same type of wine. The quality index Q was calculated for the five TMRs. The two FUs derived from Q were sensitive to a change of quality target. The LCA results of the five TMRs expressed per MQFU differed from results per QFU and were sensitive to a change of quality target. Results with QFU were close to those obtained with classical FUs (1 kg grapes and 1 ha vines, 1 year) due to minor differences in the value of Q. QFU appeared too restrictive while MQFU allowed accounting for the main function of the system : the production of a given quantity of quality grapes with a target of a given type of wine.