Reproductive effects of gestational exposure to diesel exhaust in a rabbit model
Sarah Valentino
(1, 2)
,
Anne Couturier-Tarrade
(1, 2)
,
Delphine Rousseau-Ralliard
(1, 2)
,
Eve Mourier
(1, 2)
,
Christophe Richard
(1, 2)
,
Michèle Dahirel
(1, 2)
,
Josiane Aioun
(1, 2)
,
Nathalie Daniel
(1, 2)
,
Catherine Archilla
(1, 2)
,
Nathalie Peynot
(1, 2)
,
Natalie Fournier
(3)
,
Marine Guinot
(1, 2)
,
Geneviève Jolivet
(1, 2)
,
Céline Faure
(4)
,
Mathilde Bourdon
(1, 2)
,
Laura Torres Rovira
(1, 2)
,
Luc Jouneau
(1, 2)
,
Madia Charlier
(5)
,
Etienne Aujean
(5)
,
John Boere
(6)
,
Lydiane Agier
(7)
,
Flemming R. Cassee
(8)
,
Rémy Slama
(7)
,
Pascale Chavatte-Palmer
(1, 2)
1
BDR -
Biologie du Développement et Reproduction
2 Université Paris Saclay (COmUE)
3 Groupe de Chimie Analytique de Paris-Sud
4 CHU Tenon [AP-HP]
5 GABI - Génétique Animale et Biologie Intégrative
6 RIVM - National Institute for Public Health and the Environment [Bilthoven]
7 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
8 Centre for Sustainability Environment and Health
2 Université Paris Saclay (COmUE)
3 Groupe de Chimie Analytique de Paris-Sud
4 CHU Tenon [AP-HP]
5 GABI - Génétique Animale et Biologie Intégrative
6 RIVM - National Institute for Public Health and the Environment [Bilthoven]
7 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
8 Centre for Sustainability Environment and Health
Anne Couturier-Tarrade
- Fonction : Auteur
- PersonId : 184872
- IdHAL : anne-couturier-tarrade
- ORCID : 0000-0003-1559-5476
- IdRef : 089056787
Delphine Rousseau-Ralliard
- Fonction : Auteur
- PersonId : 735432
- IdHAL : delphine-rousseau-ralliard
- ORCID : 0000-0003-2684-1791
- IdRef : 058639888
Eve Mourier
- Fonction : Auteur
- PersonId : 1204122
Christophe Richard
- Fonction : Auteur
- PersonId : 743773
- IdHAL : christophe-richard
- ORCID : 0000-0003-1488-4815
Michèle Dahirel
- Fonction : Auteur
- PersonId : 746065
- IdHAL : michele-dahirel
Josiane Aioun
- Fonction : Auteur
- PersonId : 1204123
Nathalie Daniel
- Fonction : Auteur
- PersonId : 737273
- IdHAL : nathalie-daniel
- ORCID : 0000-0002-2251-1296
Catherine Archilla
- Fonction : Auteur
- PersonId : 745298
- IdHAL : catherine-archilla
Nathalie Peynot
- Fonction : Auteur
- PersonId : 744406
- IdHAL : nathalie-peynot
- ORCID : 0000-0002-1126-4322
- IdRef : 111662249
Marine Guinot
- Fonction : Auteur
- PersonId : 1204124
Geneviève Jolivet
- Fonction : Auteur
- PersonId : 745676
- IdHAL : genevieve-jolivet
- ORCID : 0000-0002-2705-7723
Luc Jouneau
- Fonction : Auteur
- PersonId : 737348
- IdHAL : luc-jouneau
- ORCID : 0000-0002-5094-7302
- IdRef : 253132681
Madia Charlier
- Fonction : Auteur
- PersonId : 744660
- IdHAL : madia-charlier
- ORCID : 0000-0001-5107-5836
Pascale Chavatte-Palmer
- Fonction : présentateur
- PersonId : 734858
- IdHAL : pascale-chavatte-palmer
- ORCID : 0000-0002-4581-6092
- IdRef : 153497998
Résumé
Mammary development and fonction was abnormal in exposed clams. The a!veolar lumina were characterized by a marked increase in fat globules number and volume (p< 0.05) . Total fatty acid (FA) content was increased (p< 0.05) in DE-exposed rabbit milk on days 2-4 of lactation, essentially due to higher medium-chain FA concentrations. Sex-specific responses to maternai exposure were observed in offspring after weaning. In-utero exposed males hacl metabolic disorders with increased blood pressure (p=0.058) , higher glycemia (p=0.019) , lower HDL-cholesterol (p=0.025) , reduced fat-to-bocly weight ratio (p=0.011) but in creased peri-renal fat mass (p=0.026). Females had only higher triglycerides (TG) compared to contrai offspring. Testicular and ovarian structure and fonction were analyzed by histology and immunohistochemistry, demonstrating no difference between groups. In contrast , there was more DN A fragmentation (p=0.0012) in epididymal sperm in the exposed group. Adult female offspring were bred with contrai males. Although fetoplacental biometry was not affected near term, 2nd generation fetal metabolism was modifiecl by grand-dam exposure with decreased plasma cholesterol (p=0.008) and increased TG concentrations (p=0.015). In conclusion, repeated gestational exposure to DE at levels close to urban pollution can affect feto-placental clevelopment and health over 2 generations.