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Poster communications

Recherche de biomarqueurs urinaires du moment optimal d’exposition à l’effet mâle

Abstract : Effective methods for synchronizing oestrus in gilts are crucial for implementation of batch management and optimum reproductive performances. Altrenogest treatments are used on a routine basis in pig farms, but there is growing demand for alternative non-hormonal breeding tools. Before puberty, gilts exhibit a “waiting period”, related to ovarian development and gonadotrophin secretions, during which urinary oestrone concentration increase. During this “waiting period”, an external stimulation, such as boar exposure, could induce the first ovulation. As non-invasive tools are required to increase knowledge about the “waiting period”, the aim of this work is to search for specific biomarkers of this period in urine. Trans-abdominal ultrasonographies were carried out for 5 weeks in six 140 days old Large White gilts until puberty detection (week -5 to week -1 before puberty). Urinary samples were collected for oestrone assay and metabolome analysis using 1H Nuclear Magnetic Resonance. Gilts were then slaughtered 7 days after puberty detection for puberty confirmation. Urinary oestrone concentration increases from week -2 to the day of puberty detection. Metabolome analysis allows the identification of 78 spectral bins, 8 of them showing significant differences between weeks. Metabolites whose concentration significantly increase or decrease during the “waiting period” (such as Trigonelline, N-acetyl-X…) could be interesting biomarkers of this period. These results confirm that non invasive urinary samples could make it possible to detect optimal time for application of boar effect. Potential urinary biomarkers of this period have been identified. This could contribute to decreasing the number of females mated while they are pre-pubertal.
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Poster communications
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https://hal.inrae.fr/hal-02743464
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Submitted on : Wednesday, June 3, 2020 - 5:59:36 AM
Last modification on : Friday, May 6, 2022 - 2:22:11 PM
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  • HAL Id : hal-02743464, version 1
  • PRODINRA : 353087

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Ghylène Goudet, Cécile Douet, Lydie Nadal-Desbarats, Nassima Haifi, Christophe Staub, et al.. Recherche de biomarqueurs urinaires du moment optimal d’exposition à l’effet mâle. 48. Journées de la Recherche Porcine, Feb 2016, Paris, France. IFIP - Institut du Porc, Journées de la Recherche Porcine en France, 2016, 48èmes Journées de la Recherche Porcine. ⟨hal-02743464⟩

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