Skip to Main content Skip to Navigation
Conference papers

Beta-carotene act as a regulator of skeletal muscle cells through BCMO1

Abstract : The enzyme beta, beta-carotene-15, 15’-monooxygenase (BCMO1) cleaves provitamin A carotenoids into active vitamin A principally in liver and intestine. The BCMO1 gene is expressed at low level in the muscle tissue, including in myoblasts, but little is known about its function. In the chicken muscle, we observed that various bcmo1 expression levels are associated with different carotenoids contents. To investigate the potential role of BCMO1 on skeletal muscle, we assessed the impact of beta-carotene (BC), the prototype substrate of the BCMO1 enzyme, supplementation in vitro on proliferative primary avian myoblasts. Proliferation was evaluated by BrdU incorporation and by flow cytometry. The BrdU incorporation index was reduced and the proportion of G0/G1 cells increased following BC supplementation. Cell differentiation was evaluated by immunolabelling of sarcomeric myosin heavy chain (MHC). The proportion of sarcomeric MHC expressing cells and the differentiation index increased following BC supplementation despite the proliferative environment. The effects of BC were inhibited in the presence of DEAB, an inhibitor of retinaldehyde dehydrogenase. These results are in accordance with the hypothesis that the BCMO1 enzyme is active in myoblasts and can contribute to the retinoic acid production from BC. These data suggest that provitamin A could be used as a potential nutritional regulator of skeletal muscle growth.
Document type :
Conference papers
Complete list of metadata
Contributor : Migration ProdInra Connect in order to contact the contributor
Submitted on : Wednesday, June 3, 2020 - 6:44:47 AM
Last modification on : Tuesday, January 11, 2022 - 5:56:24 PM


  • HAL Id : hal-02743919, version 1
  • PRODINRA : 309915


Christophe Praud, Sultan Al Ahmadieh, Yves Le Vern, Cécile Berri, Michel Jacques M.J. Duclos. Beta-carotene act as a regulator of skeletal muscle cells through BCMO1. 22. European Symposium on the Quality of Poultry Meat - 16. European Symposium on the Quality of Eggs and Egg Products, World's Poultry Science Association (WPSA). INT., May 2015, Nantes, France. 218 p. ⟨hal-02743919⟩



Record views