Validation of a new rabbit microarray: transcriptome variation in PBMCs after in vitro stimulation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2013

Validation of a new rabbit microarray: transcriptome variation in PBMCs after in vitro stimulation

Abstract

Due to both physiological and economic reasons, rabbits have proven very useful as biomedical models for humans. However, genome-wide expression studies targeting the immune response are still limited in this species. We have designed a long oligonucleotide-based DNA microarray by combining a generic Agilent rabbit gene expression microarray with missing well-annotated genes known to be involved in immune and inflammatory responses. Rabbit peripheral blood mononuclear cells (PBMCs) were isolated and either mock-stimulated or stimulated with lipopolysaccharide (LPS) or a mixture of phorbol myristate (PMA) and ionomycin for 4 (T4) and 24 (T24) h. After statistical analyses using LIMMA package in R, we have shown that the number of differentially expressed genes after PMA/ionomycin stimulation was 20 and 24 times higher at T4 and T24, respectively, compared to the LPS stimulation at similar time points. At T4, LPS stimulation induced an over-expression (FDR<0.05) of pro-inflammatory (IL23A) or regulatory (ILI2A) cytokines and chemokine molecules (CXCLIl), but a dawn-regulation of the LPS receptor CD14. At T24 , ILIB, IL10, IL23A and IL36G were found over-expressed. At T4, PMA/ionomycin induced both Th1 (IL2 and IL1O) and Th2 responses (IL4 and IL13). NFKBID and TBX21 were over-expressed whereas IL16, LTB and CD79B were found significantly down-regulated. At T24, IL2 and IL4 were still found over­ expressed after PMA/ionomycin stimulation whereas IL1R2 and ILlB were down-regulated. Part of our results agrees with a similar previous report on the transcriptome of pig PBMCs. A microarray enriched in immunity-related genes is now available and can be used as a new tool for genome-wide expression studies of immunity in the rabbit species.
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Dates and versions

hal-02747487 , version 1 (03-06-2020)

Identifiers

  • HAL Id : hal-02747487 , version 1
  • PRODINRA : 226787

Cite

Vincent Jacquier, Jordi Estellé, Barbara B. Panneau, Jerôme J. Lecardonnel, Marco M. Moroldo, et al.. Validation of a new rabbit microarray: transcriptome variation in PBMCs after in vitro stimulation. 64. Annual Meeting of the European Federation of Animal Science (EAAP), European Association for Animal Production (EAAP). ITA., Aug 2013, Nantes, France. 660 p. ⟨hal-02747487⟩
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