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Poster communications

Arbuscular mycorrhizae influence the occurence of soil fluorescent pseudomonads harbouring type III secretion systems

Abstract : Type III secretion systems (T3SS) of pathogenic and symbiotic Gram negative bacteria allow direct interactions with eukaryotic cells. Saprophytic fluorescent Pseudomonas spp. harbouring T3SS genes (T3SS+) have been more frequently encountered in the rhizosphere than in bulk soil, which suggests a possible interaction between these bacteria and rhizospheric eukaryotes.Our objectives were to compare, (i) the effect of mycorrhizal and of non-mycorrhizal roots of Medicago truncatula on the occurrence and diversity of T3SS+ fluorescent Pseudomonas spp.and (ii) the diversity of T3SS+and T3SS- bacterial genotypes.Fluorescent pseudomonads from (i) the rhizosphere of mycorrhizal roots of M. truncatula Jemalong J5 (wild-type, Myc+ Nod+) and TRV48 (mutant Myc+ Nod-), (ii) the rhizosphere of non-mycorrhizal roots of TRV45 (mutant Myc- Nod-), and (iii) the corresponding bulk soil were isolated. Pseudomonas spp. strains harbouring T3SS genes were identified by PCR targeting a conserved hrcRST DNA fragment. Genotypic diversity of fluorescent pseudomonads has been described by a whole cell rep-PCR fingerprinting method (BOX-PCR) and 16S rRNA sequencing. For T3SS+ strains the polymorphism of hrcRST has been assessed by PCR-RFLP and sequencing.The occurrence of T3SS+ fluorescent pseudomonads was significantly higher in the rhizosphere of mycorrhizal (J5 and TRV48) than in that of non-mycorrhizal roots (TRV45) or in the bulk soil. Four distinct hrcRST genotypes were described, two of which were specifically described for strains associated with mycorrhizal roots. Compared to hrcRST negative fluorescent pseudomonads, T3SS+ strains displayed specific BOX-PCR genotypes that were all ascribed to the phylogenetic group of P. fluorescens on the basis of 16S rRNA gene identity.Mycorrhizal roots of Medicago truncatula clearly influence the population structure of root associated fluorescent pseudomonads in a different way than non-mycorrhizal roots. The occurrence of particular genotypes of fluorescent Pseudomonas spp. harbouring T3SS genes was highly increased in the rhizosphere of mycorrhizal roots suggesting that T3SS might be implicated in cellular interactions between fluorescent pseudomonads and arbuscular mycorrhizae.
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Poster communications
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https://hal.inrae.fr/hal-02755399
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  • HAL Id : hal-02755399, version 1
  • PRODINRA : 39025

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Amandine Viollet, Thérèse Corberand, Adeline Robin, Christophe Mougel, Philippe Lemanceau, et al.. Arbuscular mycorrhizae influence the occurence of soil fluorescent pseudomonads harbouring type III secretion systems. Bageco 10 - Bacterial genetics and ecology, Jun 2009, Uppsala, Sweden. 1p., 2009. ⟨hal-02755399⟩

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