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Communication dans un congrès

Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI

Abstract : Lipid droplets (LDs) and mitochondria in the ooplasm are essential for energy production required for maturation, fertilization and embryo development. This Study investigates the correlations between cytoplasmic LDs polar aggregation and: (1) nuclear maturation (Experiment 1): (2) mitochondrial (nit) distribution pattern and localization (Experiment 2): (3) fertilization and embryonic development after intracytoplasmic sperm injection (ICSI; Experiment 3) in equine oocytes recovered from slaughtered mares and matured in vitro. Morphologically normal oocytes were selected after culture and categorized as having polar (P) aggregation or uniform (U) distribution of LDs. In Experiment 1. the maturation rate was significantly higher in P compared with U oocytes (69%, 40/58 vs. 32%, 13/41: P < 0.001). In Experiment 2. it was observed that P and U oocytes showed heterogeneous mt distribution at comparable rates (68%, 25/37 vs. 50%, 2/4 for P and U respectively; NS). Moreover, only in 8/25 (32%) of P oocytes. LDs overlapped with nit aggregates in the area containing meiotic spindle. In Experiment 3. normal fertilization (51%, 19/37 vs. 60%. 6/10. for P and U) and cleavage rates (83%, 20/24 vs. 67%, 4/6, for P and U) did not differ between groups, also in oocytes with LDs located nearby the polar body. Overall, P aggregation of LDs was related to cumulus of expansion at collection. In conclusion, in equine matured oocytes. P aggregation of LDs is related with cumulus expansion and nuclear maturation. However, it is not related with heterogeneous mt distribution and cannot be considered a predictive indicator for normal fertilization and embryo.
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Communication dans un congrès
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https://hal.inrae.fr/hal-02758521
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 03:49:40
Dernière modification le : vendredi 5 février 2021 - 03:51:47

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Barbara Ambruosi, G.M. Lacalandra, A.I. Iorga, Teresa de Santis, Sylvie Mugnier, et al.. Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI. 16. International Congress on Animal Reproduction, Jul 2008, Budapest, Hungary. ⟨10.1016/j.theriogenology.2008.12.002⟩. ⟨hal-02758521⟩

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