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Poster communications

Steroidome and metabolome analysis in gilt saliva to identify biomarkers of boar effect receptivity

Ghylene Goudet 1 Philippe Liere 2 Lydie Nadal-Desbarats 3 Doryan Grivault 4 Cécile Douet 1 Jonathan Savoie 5 Stéphane Ferchaud 4 Florence Maupertuis 6 Antoine Roinsard 7 Sylviane Boulot 8 Armelle Prunier 9
1 PRC - Physiologie de la reproduction et des comportements [Nouzilly]
2 Petites Molécules de neuroprotection, neurorégénération et remyélinisation
3 UT - Université de Tours
4 GenESI - Génétique, Expérimentation et Système Innovants
5 UE PAO - Unité Expérimentale de Physiologie Animale de l‘Orfrasiére
6 Chambre Régionale d'Agriculture des Pays de la Loire
7 ITAB - Institut Technique de l'Agriculture Biologique
8 IFIP - Institut du Porc
9 PEGASE - Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes]
Abstract : Our objective was to develop alternatives to hormonal treatments to synchronize oestrus of gilts. Before puberty gilts exhibit a pre-puberty period during which boar exposure could induce and synchronize first ovulation. To develop practical non-invasive tools to identify this period and improve detection of the gilts to stimulate, we searched for salivary biomarkers of the pre-puberty period. Saliva samples were collected from 30 Large-White x Landrace crossbred gilts from 140 to 175 days of age. Gilts were exposed to a boar twice a day and subjected to oestrus detection from 150 to 175 days of age. Among the 30 gilts, 10 were detected in oestrus 4 to 7 days after introduction of the boar and were considered receptive to the boar effect, 14 were detected in oestrus more than 8 days after boar introduction, 6 did not show oestrus and were considered non-receptive. Saliva samples from 6 receptive and 6 non-receptive gilts were analysed for steroidome using GC-MS/MS and for metabolome using 1H-NMR spectroscopy. Four saliva samples per gilt were analysed: 26 days and 11 days before boar introduction (BI-26 and BI-11), the day of boar introduction (BI), 3 days later for receptive gilts (BI+3) or 7 days later for non-receptive gilts (BI+7). Data were analysed using repeated measures one-way ANOVA and orthogonal partial least squares discriminant analysis. Thirty steroids and 35 metabolites were detected in gilt saliva. The concentrations of 6 steroids were higher (P < 0.05) in receptive gilts than in non-receptive gilts at BI-26, BI-11 and BI. The concentration of 2 metabolites were lower (P < 0.05) in receptive gilts than in non-receptive gilts at BI-11. These candidates could be potential salivary biomarkers to detect receptive gilts. However, their low and variable concentrations in saliva require expensive analysis and limit their use in pig farms.
Keywords : biomarkers identification
Document type :
Poster communications
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https://hal.inrae.fr/hal-02787200
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Submitted on : Friday, June 5, 2020 - 1:34:13 AM
Last modification on : Friday, May 6, 2022 - 2:22:12 PM

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  • HAL Id : hal-02787200, version 1
  • PRODINRA : 475314

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INRAE | INRA | UNAM | UNIV-TOURS | CNRS | UNIV-PARIS-SACLAY | PHASE | INRAENOUVELLEAQUITAINEPOITIERS

Citation

Ghylene Goudet, Philippe Liere, Lydie Nadal-Desbarats, Doryan Grivault, Cécile Douet, et al.. Steroidome and metabolome analysis in gilt saliva to identify biomarkers of boar effect receptivity. 70th annual meeting of the European Federation of Animal Science (EAAP), Aug 2019, Ghent, Belgium. 2019. ⟨hal-02787200⟩

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