Genome sequence of the cluster root forming white lupin;

Barbara Hufnagel; André Marques; Alexandre Soriano; Laurence Marquès; Fanchon Divol; Patrick Doumas; Erika Sallet; Davide Mancinotti; Sébastien Carrere; William Marande; Sandrine Arribat; Jean Keller; Cécile Huneau; Thomas Blein; Delphine Aime; Malika Laguerre; Jemma Taylor; Veit Schubert; Matthew Nelson; Fernando Geu-Flores; Martin Crespi; Karine Gallardo-Guerrero; Pierre-Marc Delaux; Jerome Salse; Helene Berges; Romain Guyot; Jerome Gouzy; Benjamin Peret

doi:10.1101/708917

Preprints, Working Papers, ...

Genome sequence of the cluster root forming white lupin;

Barbara Hufnagel ¹ André Marques ¹ Alexandre Soriano ¹ Laurence Marquès ¹ Fanchon Divol ¹ Patrick Doumas ¹ Erika Sallet ² Davide Mancinotti ³ Sébastien Carrere ² William Marande ⁴ Sandrine Arribat ⁴ Jean Keller ⁵ Cécile Huneau ⁶ Thomas Blein ⁷ Delphine Aime ⁸ Malika Laguerre ¹ Jemma Taylor ⁹ Veit Schubert ¹⁰ Matthew Nelson ^{9, 11} Fernando Geu-Flores ³ Martin Crespi ⁷ Karine Gallardo-Guerrero ⁸ Pierre-Marc Delaux ¹² Jerome Salse ⁶ Helene Berges ⁴ Romain Guyot ¹³ Jerome Gouzy ² Benjamin Peret ^{1, *}

* Corresponding author

1 BPMP - Biochimie et Physiologie Moléculaire des Plantes

2 LIPM - Laboratoire des interactions plantes micro-organismes

3 KU - University of Copenhagen = Københavns Universitet

4 CNRGV - Centre National de Ressources Génomiques Végétales

5 LRSV - Laboratoire de Recherche en Sciences Végétales

6 GDEC - Génétique Diversité et Ecophysiologie des Céréales

7 IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay

8 Agroécologie [Dijon]

9 Royal Botanic Gardens, Kew

10 Leibniz Institute of Plant Genetics and Crop Plant Research

11 CSIRO - Commonwealth Scientific and Industrial Research Organisation [Canberra]

12 Evolution des Interactions Plantes-Microorganismes

LRSV - Laboratoire de Recherche en Sciences Végétales

13 IRD - Institut de Recherche pour le Développement

Abstract : White lupin (Lupinus albus L.) is a legume that produces seeds recognized for their high protein content and good nutritional value (lowest glycemic index of all grains, high dietary fiber content, and zero gluten or starch)1–5. White lupin can form nitrogen-fixing nodules but has lost the ability to form mycorrhizal symbiosis with fungi6. Nevertheless, its root system is well adapted to poor soils: it produces cluster roots, constituted of dozens of determinate lateral roots that improve soil exploration and phosphate remobilization7. As phosphate is a limited resource that comes from rock reserves8, the production of cluster roots is a trait of interest to improve fertilizers efficiency. Using long reads sequencing technologies, we provide a high-quality genome sequence of a modern variety of white lupin (2n=50, 451 Mb), as well as de novo assemblies of a landrace and a wild relative. We describe how domestication impacted soil exploration capacity through the early establishment of lateral and cluster roots. We identify the APETALA2 transcription factor LaPUCHI-1, homolog of the Arabidopsis morphogenesis coordinator9, as a potential regulator of this trait. Our high-quality genome and companion genomic and transcriptomic resources enable the development of modern breeding strategies to increase and stabilize yield and to develop new varieties with reduced allergenic properties (caused by conglutins10), which would favor the deployment of this promising culture.

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Preprints, Working Papers, ...

Domain :

Life Sciences [q-bio] / Vegetal Biology

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Submitted on : Friday, June 5, 2020 - 6:32:17 AM
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HufnagelB.-et al-BioRxiv-2019_...

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