The first set of expressed sequence TAGS (ESTS) in the medicinal mushroom Agaricus subrufescens provides opportunitgies to discover production pathways of bioactive compounds and develop molecular breeding - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Poster De Conférence Année : 2014

The first set of expressed sequence TAGS (ESTS) in the medicinal mushroom Agaricus subrufescens provides opportunitgies to discover production pathways of bioactive compounds and develop molecular breeding

Résumé

Agaricus subrufescens Peck is one of the most important culinary-medicinal cultivable mushrooms with potentially high added-value products and extended agronomical valorization. It has been reported to produce various bioactive compounds that could have potential medicinal applications. However, no study dedicated to identify the underlying molecular mechanisms has been reported yet due, among others, to a lack of genomic resources. To fill this gap, we have developed the first set of expressed sequence tagged (EST) fragments available for A. subrufescens using 454 pyrosequencing technology. Using one quarter-plate pyrosequencing run for two tagged samples, we obtained about 30 Mb of raw data per sample. The assembly process resulted in two sets of 4989 and 5125 unigenes respectively. Using BLASTx program, most of the sequences (80%) found significant hit against non-redundant protein database and 87% showed similarity with A. bisporus predicted protein. The assignation of Gene Ontology (GO) terms was possible for 50 % of the sequences. We explored in silico the assembled sequences to identify putative genes potentially involved in production pathways of bioactive compounds. EST sequences were also used as a source of molecular markers and new microsatellite markers were developed. The new EST dataset provides solid genomic bases for decoding the metabolic engineering as well as efficient tools to foresee genetic studies and molecular breeding of A. subrufescens. It offers also opportunities to initiate comparative genomic studies in Agaricus species, and to a larger extent in basidiomycetes.
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Dates et versions

hal-02797498 , version 1 (05-06-2020)

Identifiants

  • HAL Id : hal-02797498 , version 1
  • PRODINRA : 257249

Citer

Marie Foulongne-Oriol, Nicolas Lapalu, Joelle J. Amselem, Jean-Michel Savoie. The first set of expressed sequence TAGS (ESTS) in the medicinal mushroom Agaricus subrufescens provides opportunitgies to discover production pathways of bioactive compounds and develop molecular breeding. 12th European conference on fungal genetics, Mar 2014, Séville, Spain. , p.315, 2014. ⟨hal-02797498⟩
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