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Conference papers

Long term effects of environmental EDC exposure on ovine pituitary KiSS-1 expression.

Abstract : Environmental exposure to endocrine disrupting compounds (EDCs) during development could adversely affect reproductive function via effects on the hypothalamo-pituitary-gonadal axis (HPG). The HPG axis is regulated by many steroid sensitive inputs, including the KiSS-1 gene product kisspeptin. We have previously shown that KiSS-1 mRNA and protein are expressed within gonadotrophs in the foetal ovine pituitary and that KiSS-1 mRNA expression is decreased following gestational exposure to EDCs. The current study aimed to determine whether KiSS-1 mRNA expression is also changed in pituitary glands of adult animals exposed to EDCs in-utero. Tissue was obtained from 12 month old male and female sheep born to mothers pastured on fields fertilised with inorganic fertiliser (C) or sewage sludge (T) throughout pregnancy (n=12 per group). mRNA was extracted, reverse transcribed and KiSS-1 mRNA expression quantified by QrtPCR (relative to β-actin). Data were analysed using two-way ANOVA. Results - KiSS-1 mRNA expression was significantly (P<0.0001) greater in pituitary glands of male compared to female animals. KiSS-1 mRNA expression was significantly (P<0.05) decreased in T compared to C animals, regardless of gender. The observation of difference in KiSS-1 expression between genders in adult sheep, contrasts with our previous results but agrees with previous reports that gender specific differences in KiSS-1 develop postnatally. Interestingly, the results demonstrate EDC induced changes in foetal pituitary KiSS-1 expression are maintained into adulthood. These results support the possibility that the KiSS-1 system could be programmed by developmental EDC exposure and affect later reproductive function.
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Submitted on : Saturday, June 6, 2020 - 1:59:05 PM
Last modification on : Wednesday, November 24, 2021 - 9:54:15 AM


  • HAL Id : hal-02816521, version 1
  • PRODINRA : 39062



Michelle Bellingham, Paul A. Fowler, Richard M. Sharpe, Maria R. Amezaga, Béatrice Mandon-Pepin, et al.. Long term effects of environmental EDC exposure on ovine pituitary KiSS-1 expression.. BSN MEETING 2009, Jun 2009, Edinburgh, United Kingdom. n.p. ⟨hal-02816521⟩



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