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Journal Articles Journal of Mass Spectrometry Year : 2017

Myofiber metabolic type determination by mass spectrometry imaging

Delphine Centeno
Estelle Pujos-Guillot
Thierry Astruc
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Laetitia Theron

Abstract

Matrix assisted laser desorption/ ionization (MALDI) mass spectrometry imaging is a powerful tool that opens new research opportunities in the field of biology. In thiswork, predictive modelwas developed to discriminatemetabolic myofiber types using the MALDI spectral data. Rat skeletal muscles are constituted of type I and type IIA fiber, which have an oxidative metabolism for glycogen degradation, and type IIX and type IIB fiber which have a glycolytic metabolism, present in different proportions according to the muscle function and physiological state. So far, myofiber type is determined by histological methods that are time consuming. Thanks to the predictive model, we were able to predict not only the metabolic fiber type but also their location, on the same muscle section that was used for MALDI imaging.

Dates and versions

hal-02903088 , version 1 (20-07-2020)

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Delphine Centeno, Annie A. Venien, Estelle Pujos-Guillot, Thierry Astruc, Christophe C. Chambon, et al.. Myofiber metabolic type determination by mass spectrometry imaging. Journal of Mass Spectrometry, 2017, 52 (8), pp.493-496. ⟨10.1002/jms.3957⟩. ⟨hal-02903088⟩
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