Skip to Main content Skip to Navigation
Journal articles

Surface properties associated with the production of polysaccharides in the food bacteria Propionibacterium freudenreichii

Abstract : This study explores the production of polysaccharides (PS) in the strain Pf2289 of the food species Propioni-bacterium freudenreichii. Pf2289 presents characteristics atypical of the species: a molar-shaped morphotype upon plating, and cells strongly aggregative in liquid medium. When plating Pf2289, another morphotype was observed with a 4% frequency of appearance: round-shaped colonies, typical of the species. A clone was isolated, designated Pf456. No reversibility of Pf456 towards the molar-shaped morphotype was observed. Pf2289 was shown to produce a surface polysaccharide (PS) bound to the cell wall, mainly during the stationary growth phase. Meanwhile, Pf456 had lost the ability to produce the PS. AFM images of Pf2289 showed that entangled filaments spread over the whole surface of the bacteria, whereas Pf456 exhibited a smooth surface. Adhesion force maps, performed with concanavalin-A grafted probes, revealed twice as much adhesion of Pf2289 to concanavalin-A compared to Pf456. Furthermore, the length of PS molecules surrounding Pf2289 measured at least 7 μm, whereas it only reached 1 μm in Pf456. Finally, the presence of PS had a strong impact on adhesion properties: Pf2289 did not adhere to hydrophobic surfaces, whereas Pf456 showed strong adhesion.
Document type :
Journal articles
Complete list of metadata

Cited literature [56 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02911595
Contributor : Anne Giboulot <>
Submitted on : Tuesday, August 4, 2020 - 9:38:42 AM
Last modification on : Tuesday, September 21, 2021 - 3:23:47 AM
Long-term archiving on: : Monday, November 30, 2020 - 1:59:11 PM

File

2020-FoodMicro-Guyomarc'h-Pf-E...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Fanny Guyomarc'H, Gregory Francius, Sandrine Parayre, Marie-Noelle Madec, Stéphanie-Marie Deutsch. Surface properties associated with the production of polysaccharides in the food bacteria Propionibacterium freudenreichii. Food Microbiology, Elsevier, 2020, 92, pp.103579. ⟨10.1016/j.fm.2020.103579⟩. ⟨hal-02911595⟩

Share

Metrics

Record views

39

Files downloads

180