Fat‐Shaped microbiota affects lipid metabolism, Liver Steatosis, and intestinal homeostasis in mice fed a low‐protein diet - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Molecular Nutrition and Food Research Year : 2020

Fat‐Shaped microbiota affects lipid metabolism, Liver Steatosis, and intestinal homeostasis in mice fed a low‐protein diet

Catherine Philippe
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Abstract

Scope Protein malnutrition is characterized by stunted growth, hepatic steatosis and a damaged gut mucosal architecture. Since high-fat shaped gut microbiota (HFM) has an increased ability in providing nutrients and energy from food to the host, the aim of this study is to determine whether such a microbiota could beneficially impact on the consequences of malnutrition. Methods and results The cecal content of specific pathogen free C57Bl/6J mice fed a high-fat diet or a low-protein diet is transplanted in two groups of germ-free C57Bl/6J recipient mice, which are subsequently fed a low-protein diet for 8 weeks. Body weight gain is comparable between the two groups of microbiota-recipient mice. The HFM led to a worsening of microvesicular steatosis and a decrease of plasma lipids compared to the low-protein shaped microbiota. In the small intestine of mice receiving the HFM, although significant histological differences are not observed, the expression of antimicrobial genes promoting oxidative stress and immune response at the ileal epithelium (Duox2, Duoxa2, Saa1, Ang4, Defa5) is increased. Conclusion The transplant of HFM in mice fed a low-protein diet represents a noxious stimulus for the ileal mucosa and impairs hepatic lipoprotein secretion, favoring the occurrence of hepatic microvesicular steatosis.

Dates and versions

hal-02927462 , version 1 (01-09-2020)

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Marco Busnelli, Stefano Manzini, Amin Jablaoui, Aurelia Bruneau, Aicha Kriaa, et al.. Fat‐Shaped microbiota affects lipid metabolism, Liver Steatosis, and intestinal homeostasis in mice fed a low‐protein diet. Molecular Nutrition and Food Research, 2020, 64 (15), pp.1900835. ⟨10.1002/mnfr.201900835⟩. ⟨hal-02927462⟩
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