The wild grape genome sequence provides insights into the transition from dioecy to hermaphroditism during grape domestication - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Genome Biology Année : 2020

The wild grape genome sequence provides insights into the transition from dioecy to hermaphroditism during grape domestication

Amandine Velt
Vincent Dumas
Eric Duchene
Philippe Hugueney

Résumé

Background: A key step in domestication of the grapevine was the transition from separate sexes (dioecy) in wild Vitis vinifera ssp. sylvestris (V. sylvestris) to hermaphroditismin cultivated Vitis vinifera ssp. sativa (V. vinifera). It is known that V. sylvestris has an XY system and V. vinifera a modified Y haplotype (Yh) and that the sex locus is small, but it has not previously been precisely characterized. Results: We generate a high-quality de novo reference genome for V. sylvestris, onto which we map whole-genome re-sequencing data of a cross to locate the sex locus. Assembly of the full X, Y, and Yh haplotypes of V. sylvestris and V. vinifera sex locus and examining their gene content and expression profiles during flower development in wild and cultivated accessions show that truncation and deletion of tapetum and pollen development genes on the X haplotype likely causes male sterility, while the upregulation of a Y allele of a cytokinin regulator (APRT3) may cause female sterility. The downregulation of this cytokinin regulator in the Yh haplotype may be sufficient to trigger reversal to hermaphroditism. Molecular dating of X and Y haplotypes is consistent with the sex locus being as old as the Vitis genus, but the mechanism by which recombination was suppressed remains undetermined. Conclusions: We describe the genomic and evolutionary characterization of the sex locus of cultivated and wild grapevine, providing a coherent model of sex determination in the latter and for transition from dioecy to hermaphroditism during domestication.
Fichier principal
Vignette du fichier
2020_badouin_genome_biology.pdf (1.79 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02946414 , version 1 (23-09-2020)

Licence

Paternité

Identifiants

Citer

Hélène E Badouin, Amandine Velt, François Gindraud, Timothée Flutre, Vincent Dumas, et al.. The wild grape genome sequence provides insights into the transition from dioecy to hermaphroditism during grape domestication. Genome Biology, 2020, 21 (1), pp.223. ⟨10.1186/s13059-020-02131-y⟩. ⟨hal-02946414⟩
282 Consultations
53 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More