The microbiota of the mouth disperses into the lungs, and both compartments share similar phyla. Considering the importance of the microbiota in the maturation of the immunity and physiology during the first days of life, we hypothesized that primo-colonizing bacteria of the oral cavity may induce immune responses in bronchial epithelial cells. Herein, we have isolated and characterized 57 strains of the buccal cavity of two human newborns. These strains belong to Streptococcus, Staphylococcus, Enterococcus, Rothia and Pantoea genera, with Streptococcus being the most represented. The strains were co-incubated with a bronchial epithelial cell line (BEAS-2B),and we established their impact on a panel of cytokines/chemokines and global changes in geneexpression. The Staphylococcus strains, which appeared soon after birth, induced a high productionof IL-8, suggesting they can trigger inflammation, whereas the Streptococcus strains were lessassociated with inflammation pathways. The genera Streptococcus, Enterococcus and Pantoea induceddierential profiles of cytokine/chemokine/growth factor and set of genes associated with maturationof morphology. Altogether, our results demonstrate that the microorganisms, primo-colonizingthe oral cavity, impact immunity and morphology of the lung epithelial cells, with specific eectsdepending on the phylogeny of the strains.