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Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

Abstract : The behavior of Listeria monocytogenes communities in the food chain is closely associated with their spatial organization. Whether as biofilms on industrial surfaces or as microcolonies in food matrices, the resulting physiological diversification combined with the presence of extracellular polymeric substances (EPS) triggers emergent community functions involved in the pathogen survival and persistence (e.g., tolerance to dehydration, biocides, or preservatives). In this contribution, we present a noninvasive confocal laser microscopy (CLM) protocol allowing exploration of the spatial organization of L. monocytogenes communities on various inert or nutritive materials relevant for the food industry.
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https://hal.inrae.fr/hal-03031145
Contributor : Romain Briandet Connect in order to contact the contributor
Submitted on : Monday, November 30, 2020 - 1:21:58 PM
Last modification on : Friday, August 5, 2022 - 2:38:11 PM

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Maud Darsonval, Marina Grégoire, Julien Deschamps, Romain Briandet. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities. Springer. Listeria Monocytogenes. Methods in Molecular Biology, 2220, pp.123-136, 2021, ⟨10.1007/978-1-0716-0982-8_10⟩. ⟨hal-03031145⟩

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