Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Veterinary Microbiology Year : 2021

Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France

Catherine Schouler
Pascal Galliot
  • Function : Author
Julie Puterflam
  • Function : Author

Abstract

Avian colibacillosis is the most common bacterial disease affecting broilers. To better evaluate the diversity and the origin of the causative Escherichia coli strains infecting birds, we conducted a study on 80 broiler flocks. Just before the arrival of chicks on the farm, samples were collected in the farm environment (walls, feeders, air inlets, etc.) and, upon delivery, day-old chicks (DOCs) and the transport boxes were also sampled. Isolates were obtained from these samples, and from organs of chickens exhibiting typical colibacillosis symptoms. The isolates were characterized using high-throughput qPCR to detect a range of genetic markers (phylogroups, main serogroups virulence markers, etc.). A total of 967 isolates were studied, including 203 from 28 colibacillosis episodes, 484 from DOCs, 162 from transport boxes and 118 from the farm environment. These isolates yielded 416 different genetic profiles, of which 267 were detected in single isolates, and the others were observed in up to 44 isolates from nine farms. The distributions of isolates across phylogroups and the main serogroups varied with the origin of isolation. The isolates obtained from colibacillosis cases either shared a single genetic profile or were different. In a few cases, we observed the same profile for isolates obtained from DOCs and colibacillosis lesions in the same flock or different flocks. However, some flocks receiving DOCs contaminated with isolates bearing the genetic profile of colibacillosis cases identified in other flocks remained healthy. This study highlights the huge diversity among avian E. coli isolated from diseased and non diseased birds.
Fichier principal
Vignette du fichier
S0378113520310610.pdf (499.23 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03107169 , version 1 (02-01-2023)

Licence

Attribution - NonCommercial

Identifiers

Cite

Sabine Delannoy, Catherine Schouler, Rozenn Souillard, Léna Yousfi, Laetitia Le Devendec, et al.. Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France. Veterinary Microbiology, 2021, 252, pp.108923. ⟨10.1016/j.vetmic.2020.108923⟩. ⟨hal-03107169⟩
121 View
55 Download

Altmetric

Share

Gmail Facebook X LinkedIn More