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In vitro solubilization of fat-soluble vitamins in structurally defined mixed intestinal assemblies

Abstract : The structures of fed state intestinal assemblies containing bile components, dietary fat, and fat-solublevitamins are not well known, although they are involved in lipid transport. In this study, several methodswere used to investigate structural transitions upon various dietary lipids or various fat-soluble vitaminsincorporation in bile intestinal assemblies. In particular, DLS and turbidimetry were used to study tran-sition points as a function of component concentration, and cryo-TEM and SAXS were used to resolveassembly structures at microscopic and supramolecular scales, respectively. Results showed that increas-ing the concentration of dietary lipids in bile assembly induced a transition from core-shell micelles tounilamellar vesicles (except with caprylate lipids, always yielding micelles). In these specific assemblies,increasing the concentration of a fat-soluble vitamin either induced a systematic structural transition,defining a solubilization capacity (a-tocopherol or phylloquinone), or induced a structural transition onlyin micelles (retinol), or did not induce any structural transition up to very high concentrations (cholecal-ciferol). Using SAXS data, ideal molecular organizations are proposed for assemblies in the absence orpresence ofa-tocopherol.
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Contributor : Olivier Dupre <>
Submitted on : Thursday, January 28, 2021 - 10:18:59 AM
Last modification on : Tuesday, February 2, 2021 - 1:56:46 PM
Long-term archiving on: : Thursday, April 29, 2021 - 6:23:39 PM


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Asma El Aoud, Emmanuelle Reboul, Aurélien Dupont, Cristelle Mériadec, Franck Artzner, et al.. In vitro solubilization of fat-soluble vitamins in structurally defined mixed intestinal assemblies. Journal of Colloid and Interface Science, Elsevier, 2021, 589, pp.229-241. ⟨10.1016/j.jcis.2021.01.002⟩. ⟨hal-03122063⟩



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