In addition to the major and well-known milk components, nutritional quality of milk is also influenced by recently detected components, such as miRNAs that regulate many biological processes. They were found in different fractions of milk including extracellular vesicles (EVs). Evidence of the presence of food-sourced miRNAs in human plasma suggests that they could influence human health. In goats, the polymorphisms occurring at the CSN1S1 locus deeply impact mammary epithelial cell (MEC) function and milk composition. We hypothesized that this polymorphism may influence cargo miRNA in milk-derived EVs. Our objectives were to: i) characterize the miRNome from milk-derived EVs; ii) compare the miRNomes of strong (AA) vs natural-KO (OO) genotypes and hypothesize on the putative effects on milk consumers. Total RNA isolated from EVs was subjected to RNAseq using Illumina NextSeq500. Sequences were mapped on Bos taurus genome (UMD3.1) and miRBase. Differential analyses, multiple testing correction and bioinformatics analyses were performed.