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A Combination of Single Nucleotide Polymorphisms is Associated with the Interindividual Variability of Cholesterol Bioavailability in Healthy Adult Males

Abstract : Scope Cholesterol bioavailability displays a high interindividual variability, partly due to genetic factors. Existing studies have focused on single nucleotide polymorphisms (SNPs) analyzed individually, which only explained a minor fraction of the variability of this complex phenotype. The aim is to identify a combination of SNPs associated with a significant part of the variability in cholesterol bioavailability. Methods and results Thirty-nine healthy adult males are given a standard test snack containing 80 mg heptadeuterated (D7) cholesterol. The plasma D7-cholesterol concentration is measured at equilibrium 40 h after test snack intake. The D7-cholesterol response (D7-cholesterol/total cholesterol concentration) exhibits a relatively high interindividual variability (CV = 32%). The association of exonic SNPs in candidate genes (188 genes involved in or related to cholesterol metabolism) with the plasma D7-cholesterol response is assessed by univariate statistics followed by partial least squares regression. A significant model (p-value after cross-validation ANOVA = 1.64 x 10(-7)) that includes 8 SNPs (SOAT2-rs9658625,DNAH11-rs11768670,LIPC-rs690,MVK-rs2287218,GPAM-rs10787428,APOE-rs7412,CBS-rs234706, andWRN-rs1801196) explains 59.7% of the variance in cholesterol bioavailability (adjustedR(2)). Conclusion Here a combination of SNPs is significantly associated with the variability in dietary cholesterol bioavailability in healthy adult males.
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https://hal.inrae.fr/hal-03155917
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Submitted on : Tuesday, March 9, 2021 - 11:10:48 AM
Last modification on : Tuesday, May 11, 2021 - 4:50:04 PM
Long-term archiving on: : Thursday, June 10, 2021 - 6:50:01 PM

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Charles Desmarchelier, Estelle Wolff, Catherine Defoort, Marion Nowicki, Pierre‐emmanuel Morange, et al.. A Combination of Single Nucleotide Polymorphisms is Associated with the Interindividual Variability of Cholesterol Bioavailability in Healthy Adult Males. Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2020, 64 (22), pp.2000480. ⟨10.1002/mnfr.202000480⟩. ⟨hal-03155917⟩

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