Skip to Main content Skip to Navigation
Journal articles

Control and regulation of acetate overflow in Escherichia coli

Abstract : Overflow metabolism refers to the production of seemingly wasteful by-products by cells during growth on glucose even when oxygen is abundant. Two theories have been proposed to explain acetate overflow in Escherichia coli – global control of the central metabolism and local control of the acetate pathway – but neither accounts for all observations. Here, we develop a kinetic model of E. coli metabolism that quantitatively accounts for observed behaviors and successfully predicts the response of E. coli to new perturbations. We reconcile these theories and clarify the origin, control and regulation of the acetate flux. We also find that, in turns, acetate regulates glucose metabolism by coordinating the expression of glycolytic and TCA genes. Acetate should not be considered a wasteful end-product since it is also a co-substrate and a global regulator of glucose metabolism in E. coli. This has broad implications for our understanding of overflow metabolism.
Complete list of metadata

https://hal.inrae.fr/hal-03171831
Contributor : Pierre Millard <>
Submitted on : Wednesday, March 17, 2021 - 11:22:31 AM
Last modification on : Wednesday, May 12, 2021 - 8:09:29 AM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Links full text

Identifiers

Citation

Pierre Millard, Brice Enjalbert, Sandrine Uttenweiler-Joseph, Jean-Charles Portais, Fabien Létisse. Control and regulation of acetate overflow in Escherichia coli. eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.63661⟩. ⟨hal-03171831⟩

Share

Metrics

Record views

30