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Optimization of a protein extraction method from natural rubber sheets made of Hevea brasiliensis latex

Abstract : A method to extract proteins from natural rubber (NR) samples has been developed to characterize the protein composition of NR by SDS-PAGE electrophoresis. While the incubation of NR pieces in aqueous buffers did not provide satisfactory results (no protein bands detected by SDS-PAGE), the solubilization of NR pieces in an organic solvent, prior to centrifugation and protein extraction from the obtained pellet, was found efficient to highlight protein bands by SDS-PAGE. Pure cyclohexane was used to solubilize NR pieces and resulted in a low share of nitrogen in the pellet (29%). The addition of 10% (v/v) ethanol to cyclohexane allowed to significantly increase this share to 73% and to detect 13 protein bands by SDS-PAGE. The molecular weights of the four most intense bands suggest the presence in NR of the following proteins: rubber elongation factor, small rubber particle protein, hevamine, and β-1-3 glucanase.
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https://hal.inrae.fr/hal-03197638
Contributor : Dominique Fournier <>
Submitted on : Wednesday, April 14, 2021 - 8:48:50 AM
Last modification on : Thursday, April 15, 2021 - 2:39:04 PM

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Surattiya Srisomboon, Kanthida Wadeesirisak, Laurent Vaysse, Jérôme Sainte-Beuve, Natedao Musigamart, et al.. Optimization of a protein extraction method from natural rubber sheets made of Hevea brasiliensis latex. Journal of Rubber Research, 2021, 24 (1), pp.27-39. ⟨10.1007/s42464-020-00069-1⟩. ⟨hal-03197638⟩

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